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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study; scientifically acceptable, however, no details on the test substance characteristics were given.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5,6-trimethylcyclohex-2-en-1-one
EC Number:
243-473-0
EC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Cas Number:
20030-30-2
Molecular formula:
C9H14O
IUPAC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Details on test material:
- Name of test material (as cited in study report): 2,3,6-Trimethylcyclohexen-1-one
- Analytical purity: about 95 %
water solubity: > 250 mg/l at 20 °C.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration (250 mg/l) was prepared using a 30 min stirring period at 20 °C. Then, this test medium was serially diluted with dilution water to obtain the desired test concentrations.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 24 h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
19 - 21 °C
pH:
7.4 - 8.1
Dissolved oxygen:
7.1 - 9.1 mg/l
Nominal and measured concentrations:
Nominal test concentrations (mg/l):
control, 3.91, 7.82, 15.6, 31.3, 62.6, 125.0 and 250.0
Details on test conditions:
TEST SYSTEM
- Test vessel: 20 ml flat bottom glass tubes
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: charcoal filtered and dechlorinated tap water.


OTHER TEST CONDITIONS
- Photoperiod: 16:8 hours, diffuse light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24, 48

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
62.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
62.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
83.83 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
125 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
Moving average method (Spearman-Kaerber).

Any other information on results incl. tables

No. of immobile daphnids after x hours in %:
Nominal test concentration (mg/l) 0 / 3 / 6 / 24 / 48
Control / 0 / 0 / 0 / 5 / 5
3.91 / 0 / 0 / 0 / 0 / 0
7.82 / 0 / 0 / 0 / 0 / 0
15.6 / 0 / 0 / 0 / 0 / 0
31.3 / 0 / 0 / 0 / 5 / 5
62.6 / 0 / 0 / 0 / 5 / 5
125.0 / 0 / 55 / 55 / 100 / 100
250.0 / 0 / 100 / 100 / 100 / 100

24 hour ECx values:
EC0: 62.6 mg/l
EC50: 83.83 mg/l
EC100: 125.0 mg/l

Applicant's summary and conclusion

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