Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1969
Report date:
1969

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
internal BASF method which was in large part similar to OECD Guideline 401
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5,6-trimethylcyclohex-2-en-1-one
EC Number:
243-473-0
EC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Cas Number:
20030-30-2
Molecular formula:
C9H14O
IUPAC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Details on test material:
- Name of test material (as cited in study report): 2,5,6-Trimethyl-2-cyclohexen-1-on = TMCH
- Density: 0.933 g/ml

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Gassner
- Weight at study initiation: male 156 - 260 g; female 160 - 200 g

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Traganth
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 2%, 20%, 30%
- Amount of vehicle (if gavage): 10 ml
Doses:
200, 1600, 2000, 2500, 5000 µl/kg bw (= 186.6, 1492.8, 1866, 2332.5, 4665 mg/kg bw)
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs and mortality

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 866 mg/kg bw
Mortality:
5000 µl: 17/20 deaths within 24 h; 20/20 within 48 h
2500 µl: 2/20 deaths within 24 h; 18/20 deaths within 48 h; 19/20 within 7 days
2000 µl: 1/20 deaths within 24 h; 11/20 deaths within 48 h; 11/20 deaths within 7 days
1600 µl: 5/20 deaths within 24 h; 5/20 deaths within 48 hours; 5/20 deaths within 7 days
200 µl: 0/20 deaths within 7 days
Clinical signs:
other: 2000 - 5000 µl/kg bw: - immediately after dosing staggering and high stepping gait, intermittent or accelerated respiration and salivation. - After about 10 min abdominal or lateral positions and almost unconsciousness at 2500 - 5000 µl/kg bw). - After
Gross pathology:
- tonic and dilated stomach
- almond odor of bowls
Other findings:
- Other observations: mouth smeard with blood, in some cases bloody discharge from nose, in some cases anogenital region smeared with blood

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: EU