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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1969
Report date:
1969

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
internal BASF method which was in large part similar to OECD Guideline 401
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,5,6-trimethylcyclohex-2-en-1-one
EC Number:
243-473-0
EC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Cas Number:
20030-30-2
Molecular formula:
C9H14O
IUPAC Name:
2,5,6-trimethylcyclohex-2-en-1-one
Details on test material:
- Name of test material (as cited in study report): 2,5,6-Trimethyl-2-cyclohexen-1-on = TMCH
- Density: 0.933 g/ml

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Gassner
- Weight at study initiation: male 156 - 260 g; female 160 - 200 g

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Traganth
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 2%, 20%, 30%
- Amount of vehicle (if gavage): 10 ml
Doses:
200, 1600, 2000, 2500, 5000 µl/kg bw (= 186.6, 1492.8, 1866, 2332.5, 4665 mg/kg bw)
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs and mortality

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 866 mg/kg bw
Mortality:
5000 µl: 17/20 deaths within 24 h; 20/20 within 48 h
2500 µl: 2/20 deaths within 24 h; 18/20 deaths within 48 h; 19/20 within 7 days
2000 µl: 1/20 deaths within 24 h; 11/20 deaths within 48 h; 11/20 deaths within 7 days
1600 µl: 5/20 deaths within 24 h; 5/20 deaths within 48 hours; 5/20 deaths within 7 days
200 µl: 0/20 deaths within 7 days
Clinical signs:
other: 2000 - 5000 µl/kg bw: - immediately after dosing staggering and high stepping gait, intermittent or accelerated respiration and salivation. - After about 10 min abdominal or lateral positions and almost unconsciousness at 2500 - 5000 µl/kg bw). - After
Gross pathology:
- tonic and dilated stomach
- almond odor of bowls
Other findings:
- Other observations: mouth smeard with blood, in some cases bloody discharge from nose, in some cases anogenital region smeared with blood

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: EU

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