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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD study with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1990

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-methoxy-3-nitro-N-phenylbenzamide
EC Number:
202-572-9
EC Name:
4-methoxy-3-nitro-N-phenylbenzamide
Cas Number:
97-32-5
Molecular formula:
C14H12N2O4
IUPAC Name:
4-methoxy-3-nitro-N-phenylbenzamide

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
The test item was homogenized in dilution water by ultrasonic and Ultra Turrax and then transferred into the test container

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrabärbling, Brachydanio rerio
- Strain: Hamilton Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Age at study initiation) about ten weeks:
- Length at study initiation: 2,4 - 3,6 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions: same as test
- Type and amount of food: Tetra Min, Tetra Werke. Melle; ad libitum
- Feeding frequency: twice a day


QUARANTINE (wild caught)
- Duration:
- Health/mortality:

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
no

Test conditions

Test temperature:
test group: 21,8 - 23,0 °C
control: 21,0 - 21,7
pH:
test group: 7,6 - 8,2
control: 8,0 - 8,3
Dissolved oxygen:
test group: 7,0 - 8,3 mg O2/L
control: 7,2 - 9,3 mg O2/L
Nominal and measured concentrations:
Nominal: 500 mg/L
Measured: 495 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: glass container (30 cm x 22 cm x 24 cm) calibtated to 10 liters
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Photoperiod: 12 h dark / 12 h light
- Light intensity: about 700 Lux

Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
Some fish showed decreased activity,irregular breathing, swim posture tail-heavy

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The LD50 to fish is greater than 500 mh/L
Executive summary:

The test item was tested for toxicity to fish according to OECD guideline 203.Ten Brachydanio rerio (Zebrabärbling) were exposed to a concentration of nominal 500 mg/L. No lethality was observed after 96 hours.