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Ecotoxicological information

Toxicity to terrestrial arthropods

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Administrative data

Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-03-15 - 1991-09-01
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Deviation from guideline: direct feeeding of single honeybees without anesthetization.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 213 (Honeybees, Acute Oral Toxicity Test)
Deviations:
yes
Remarks:
no anesthetization of the honeybees
GLP compliance:
yes (incl. QA statement)
Application method:
oral

Test material

Constituent 1
Chemical structure
Reference substance name:
3-methylpyrazole
EC Number:
215-925-7
EC Name:
3-methylpyrazole
Cas Number:
1453-58-3
Molecular formula:
C4H6N2
IUPAC Name:
3-methyl-1H-pyrazole

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes

Test organisms

Test organisms (species):
Apis mellifera
Animal group:
Hymenoptera (honeybees)

Study design

Study type:
laboratory study
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LD50
Effect conc.:
> 50 - < 100 µg per animal
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The acute oral LD50 of 3-Methylpyrazol to honeybees is 50-100 µg.
Executive summary:

In a 48 hr-acute oral toxicity study, honey bees (Apis mellifera L.) were exposed to 3-Methylpyrazol administered in the feed solution (0.1 ml of 1% solution of 3-Methylpyrazol in water) similar to OECD 213. The LD50 value was >50 < 100 µg a.i./bee. 

Wight of one honey bee: approx. 100 mg according to Spürgin: DIe Honigbiene, Ulmer Verlag, 2008

Based on this body weight the LD50 can be estimated with 50 microg per 100 mg bodyweight or 500 mg per kg bodyweight