Benzimidazole

Endpoint summary

Key value for chemical safety assessment

Genetic toxicity in vitro

Description of key information

Gene mutation in vitro:

Ames test:

Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical. Ames assay was performed by the preincubation protocol using Salmonella typhimurium strains  TA100, TA1535, TA1537, TA97, TA98 both in the presence and absence of S9 metabolic activation system. The study was performed at dose levels of 0, 33, 100, 333, 1000, 3333 or 6666 µg/plate following a preincubation time of 20 mins. Concurrent solvent and positive controls were included in the study. The evaluation was done considering the magnitude of the dose dependent increase in his+ revertants, and the shape of the dose response. The test chemical did not cause a dose dependent increase in the number of histidine revertants in Salmonella typhimurium strains  TA100, TA1535, TA1537, TA97, TA98 both with and witthout S9 metabolic activation system and hence is not likely to classify as a gene mutant in vitro.

Chromosome aberration test:

The test chemical 1H-benzimidazole (CAS no 51-17-2) did not induce chromosome aberrations with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

Link to relevant study records

Reference

Reference 1

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviewed publication

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

Ames Salmonella typhimurium test was conducted on Salmonella typhimurium strains TA100,TA1535,TA1537,TA97,TA98 to evaluate the mutagenic effect of the test chemical

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Target gene:

Histidine

Species / strain / cell type:

S. typhimurium, other: TA100,TA1535,TA1537,TA97,TA98

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

No data

Metabolic activation:

with and without

Metabolic activation system:

The S-9 (9,000 x g supernatant) fractions of Aroclor 1254-induced, male Sprague-Dawley rat and male Syrian hamster livers were used

Test concentrations with justification for top dose:

0, 33, 100, 333, 1000, 3333 or 6666 µg/plate

Vehicle / solvent:

DMSO

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

9-aminoacridine

sodium azide

other: 4-nitro-o-phenylenediamine (TA98 and TA1538; Without S9); 2-aminoanthracene (All strains; with S9)

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation

DURATION
- Preincubation period:20 min
- Exposure duration: 2 days (48 hrs)
- Expression time (cells in growth medium): 2 days (48 hrs)
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells):no data

SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays):No data
STAIN (for cytogenetic assays): No data

NUMBER OF REPLICATIONS: No data

NUMBER OF CELLS EVALUATED: No data

DETERMINATION OF CYTOTOXICITY No data
- Method: mitotic index; cloning efficiency; relative total growth; other: No data

OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data
- Other: No data

OTHER: No data

Rationale for test conditions:

No data

Evaluation criteria:

Magnitude of the dose dependent increase in his+ revertants, and the shape of the dose response.

Evaluations were made at both the individual trial and chemical levels. Individual trials were judged mutagenic (+), weakly mutagenic (+ W), questionable (?),
or nonmutagenic (-), depending on the magnitude of the increase in his+ revertants, and the shape of the dose response. A trial was considered questionable (?) if the dose-response was judged insufficiently high to support a call of “+ W”, if only a single dose was elevated over the control, or if a weak increase was not dose-related.

A chemical was judged mutagenic (+) or weakly mutagenic (+W) if it produced a reproducible, dose-related response over the solvent control, under a single metabolic activation condition, in replicate trials. A chemical was judged questionable (?) if the results of individual trials were not reproducible, if increases in his+ revertants did not meet the criteria for a “+W” response, or if only single doses produced increases in hisf revertants in repeat trials. Chemicals were judged nonmutagenic (-) if they did not meet the criteria for a mutagenic or questionable response.

Statistics:

No data

Species / strain:

S. typhimurium, other: TA100, TA1535, TA1537, TA97, TA98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: No data
- Effects of osmolality: No data
- Evaporation from medium: No data
- Water solubility: No data
- Precipitation: No data
- Definition of acceptable cells for analysis: No data
- Other confounding effects: No data

RANGE-FINDING/SCREENING STUDIES: Benzimmidazole was run initially in a toxicity assay to determine the appropriate dose range for the mutagenicity assay. The toxicity assay was performed using TA100or the system developed by Waleh et al. [1982]. Toxic concentrations were defined as those that produced a decrease in the number of his+ colonies, or a clearing in the density of the background lawn, or both.

CYTOKINESIS BLOCK (if used)
- Distribution of mono-, bi- and multi-nucleated cells: No data

NUMBER OF CELLS WITH MICRONUCLEI
- Number of cells for each treated and control culture: No data
- Indication whether binucleate or mononucleate where appropriate: No data

HISTORICAL CONTROL DATA (with ranges, means and standard deviation and confidence interval (e.g. 95%)
- Positive historical control data: No data
- Negative (solvent/vehicle) historical control data: No data

ADDITIONAL INFORMATION ON CYTOTOXICITY:
- Measurement of cytotoxicity used: No data
- Other observations when applicable: No data

Remarks on result:

other: No mutagenic potential

For strain TA100:

Dose	NA		10%HLI		30% HLI		10%RLI		30%RLI
µG/plate	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM
0	95	8.4	136	24.4	133	3.8	90	5.8	123	11.5
33	90	4.6
100	90	5.2	142	5.7	124	1.2	84	0.3	123	9.0
333	102	4.7	153	3.5	127	13.6	101	6.2	127	2.2
1000	110	13.9	146	4.4	125	9.2	96	4.7	114	12
3333	101	1.7	130	3.3	110	6.9	101	5.5	110	18.6
6666			104	15.7	92	2.9	87	3.7	73	3.7
POS	497	38.9	458	84.4	380	14.6	339	69.0	413	11.3

 

For strain TA1535:-

Dose	NA		10%HLI		30% HLI		10%RLI		30%RLI
µG/plate	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM
0	12	1.9	12	0.3	10	2.7	7	1.8	12	2.5
33	19	5.0
100	19	2.8	12	2	12	1.5	10	0	10	1.9
333	19	0.6	7	2	9	2.0	13	2.6	11	1.3
1000	17	1.5	9	2.7	8	0.7	9	0.7	11	2.1
3333	11	2.5	11	2.1	5	0.9	9	1.0	8	1.2
6666			5	0.9	5	1.3	7	1.8	3	1.5
POS	332	14.2	168	20.4	310	24.2	198	10.7	100	0.9

 

For strain TA1537:-

Dose	NA		10%HLI		30% HLI		10%RLI		30%RLI
µG/plate	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM
0	5	1.2	9	2.3	8	2.3	8	1.7	8	0.9
33	8	2.0
100	6	0.7	6	1.0	9	1.2	9	0.9	7	0.6
333	6	0.9	6	0.9	6	1.5	6	1.3	8	1.7
1000	8	1.5	10	2.1	6	1.9	7	2.1	9	1.7
3333	7	1.5	6	1.5	7	0.9	10	1.5	7	2.0
6666			5	2	6	0.3	5	1.7	5	2.0
POS	376	62.4	50	2.9	49	2.6	43	2.8	56	4.2

 

For strain TA97:-

Dose	NA		10%HLI		30% HLI		10%RLI		30%RLI
µG/plate	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM
0	164	12.2	166	6.1	152	7.5	177	10.7	183	9.2
33	165	6.1		12.8
100	180	4.4	174	9.8	179	5.5	191	5.2	95	6.4
333	183	9	184	2.8	178	8.2	180	15.7	190	11.7
1000	156	31.4	184	10.6	159	17.6	201	15.5	206	1.5
3333	103	3.3	171	4.0	118	2	167	16.4	99	27.0
6666			142		57	8.3	87	3.6	139	13.7
POS	986	88.5	613	20.5	396	6.8	498	3.9	440	6.9

 

 

 

 

 

 

 

 

 

For strain TA98:-

Dose	NA		10%HLI		30% HLI		10%RLI		30%RLI
µG/plate	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM	Mean	SEM
0	17	2.9	31	2.7	50	2.1	17	1.2	46	3.6
33	17	1.7
100	19	1.9	28	1.2	49	4.4	22	2.2	52	2.0
333	18	2.1	30	1.5	50	3.2	24	0.6	47	4.4
1000	17	1.9	25	1.5	49	3.3	24	4.5	53	2.3
3333	8	1.9	26	2.4	42	0.9	20	1.3	46	3.1
6666			28	1.2	32	9.1	17	3.3	31	11.7
POS	675	40.9	384	45.2	211	7.1	318	33.8	311	6.4

Conclusions:

Non-mutagenic effects were known in Ames assay by using Salmonella typhimurium of strain TA100, TA1535, TA1537, TA97, TA98 when exposed with the test chemical in preincubation method and hence it is not likely to classify as a gene mutant in vitro.

Executive summary:

Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical. Ames assay was performed by the preincubation protocol using Salmonella typhimurium strains  TA100, TA1535, TA1537, TA97, TA98 both in the presence and absence of S9 metabolic activation system. The study was performed at dose levels of 0, 33, 100, 333, 1000, 3333 or 6666 µg/plate following a preincubation time of 20 mins. Concurrent solvent and positive controls were included in the study. The evaluation was done considering the magnitude of the dose dependent increase in his+ revertants, and the shape of the dose response. The test chemical did not cause a dose dependent increase in the number of histidine revertants in Salmonella typhimurium strains  TA100, TA1535, TA1537, TA97, TA98 both with and witthout S9 metabolic activation system and hence is not likely to classify as a gene mutant in vitro.

Reference 2

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE derived based on the experimental data from structurally and functionally similar read across chemicals

GLP compliance:

not specified

Type of assay:

other: In vitro chromosomal aberration test

Target gene:

No data

Species / strain / cell type:

lymphocytes: Human peripheral blood lymphocytes

Remarks:

1

Details on mammalian cell type (if applicable):

No data

Additional strain / cell type characteristics:

not specified

Species / strain / cell type:

Chinese hamster Ovary (CHO)

Details on mammalian cell type (if applicable):

No data

Additional strain / cell type characteristics:

not specified

Cytokinesis block (if used):

No data

Metabolic activation:

with and without

Metabolic activation system:

S9-Mix

Test concentrations with justification for top dose:

1. 0, 0.33, 1.0, 3.3, 10 mM
2. without: 150, 300, 400, 500, 600, 800, 1000 µg/ml;
with: 50, 100, 200, 350, 500, 700, 1000 µg/ml

Vehicle / solvent:

1. - Vehicle(s)/solvent(s) used: Distilled water
- Justification for choice of solvent/vehicle: The test chemical was soluble in distilled water

2. No data

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

Remarks:

Distilled water

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

cyclophosphamide

mitomycin C

Remarks:

1

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

1. METHOD OF APPLICATION: In medium

DURATION
- Preincubation period: No data available
- Exposure duration: No data available
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available

SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): No data available

NUMBER OF REPLICATIONS: Duplicate

NUMBER OF CELLS EVALUATED: No data available

DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data available

OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available

OTHER: No data available.

2. No data

Rationale for test conditions:

No data

Evaluation criteria:

The cell line was observed for chromosome aberration

Statistics:

No data

Species / strain:

lymphocytes: Human peripheral blood lymphocytes

Remarks:

1

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Species / strain:

Chinese hamster Ovary (CHO)

Remarks:

2

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

1. TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: No data
- Effects of osmolality: No data
- Evaporation from medium: No data
- Water solubility: No data
- Precipitation: No data
- Other confounding effects: No data

RANGE-FINDING/SCREENING STUDIES: Test
concentrations were selected based on the results of a preliminary cytotoxicity test

COMPARISON WITH HISTORICAL CONTROL DATA: No data

ADDITIONAL INFORMATION ON CYTOTOXICITY: No data

Remarks on result:

other: No mutagenic potential

Conclusions:

The test chemical 1H-benzimidazole (CAS no 51-17-2) did not induce chromosome aberrations with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

Executive summary:

Data available for the test chemical 1H-benzimidazole (CAS no 51-17-2) was reviewed to determine the mutagenic nature of the test chemical. The studies are as mentioned below:

In vitro chromosomal aberration test was performed to determine the mutagenic nature of the test chemical. The study was performed using Human peripheral blood lymphocytes with and without S9 metabolic activation system. The test chemical was dissolved in distilled water and used at dose level of0, 0.33, 1.0, 3.3, 10 mM. Concurrent solvent and positive control plates were also included in the study. The test chemical did not induce chromosome aberrations in Human peripheral blood lymphocytes with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

In another study, an in vitro mammalian cell gene Mutation test was carried out according to OECD guideline 476 using Chinese hamster ovary cells to evaluate the genetic potential of the test chemical. In results, The test chemical did not induced any chromosomal aberrations at concentrations up to 150-1000 μg/plate and 50-1000 μg/plate in Chinese hamster ovary cells in the presence and absence of S9 metabolic activation system and hence it is not likely to classify as a gene mutant in vitro.

Based on the observations made, the test chemical 1H-benzimidazole (CAS no 51-17-2) did not induce chromosome aberrations with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (negative)

Genetic toxicity in vivo

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Gene mutation in vitro:

Various peer reviewed publications were reviewed to determine the mutagenic nature of 1H-benzimidazole (IUPAC name:1H-benzimidazole). The studies are as mentioned below:

Ames test:

Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical. Ames assay was performed by the preincubation protocol using Salmonella typhimurium strains  TA100, TA1535, TA1537, TA97, TA98 both in the presence and absence of S9 metabolic activation system. The study was performed at dose levels of 0, 33, 100, 333, 1000, 3333 or 6666 µg/plate following a preincubation time of 20 mins. Concurrent solvent and positive controls were included in the study. The evaluation was done considering the magnitude of the dose dependent increase in his+ revertants, and the shape of the dose response. The test chemical did not cause a dose dependent increase in the number of histidine revertants in Salmonella typhimurium strains  TA100, TA1535, TA1537, TA97, TA98 both with and witthout S9 metabolic activation system and hence is not likely to classify as a gene mutant in vitro.

In another study Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical. The material was dissolved in ethanol and applied at a concentration of 3 µmole/plate in the spot test performed to Salmonella typhimurium LT-2 strains TA 98, TA 100, TA 1535, and TA 1537 with and without S9 metabolic activation system. The test chemical did not induce reversion of mutant strains and hence it is not mutagenic in the bacterium Salmonella typhimurium LT-2 strains TA 98, TA 100, TA 1535, and TA 1537 with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

Gene mutation toxicity study was also performed to determine the mutagenic nature of the test chemical. The study was performed usingSalmonella typhimurium strainTA 1535, TA 1538, TA 98 and TA100 both in the presence of S9 metabolic activation system. The study was performed at dose levels of 0, 0.04, 0.1, 1, 5, or 25 mg/mL (0, 4, 20, 100, 500 or 2500µg/mL). 0.1 ml of bacterial saline suspension; 0.1 ml of solution of test compound at a given concentration in DMS0 or water; 0.15 ml of S9 mix at 4°C; 2 ml of molten top agar were mixed in bijou bottles and the contents poured over the plate to form a uniform layer, which was allowed to harden before the plates were inverted and incubated in the dark at 37°C. After 2-3 days incubation any revertant colonies were easily visible, and they were counted with an automatic colony counter which could detect colonies > 0 5 mm diameter. When colony numbers exceeded 1000 (the limit for the colony counter) numbers were estimated by visually counting a segment. The result was considered positive when there was a 2-fold increase over the negative control count for any strain. The test chemical did not induce gene mutation in Salmonella typhimurium strainTA 1535, TA 1538, TA 98 and TA100 with S9 metabolic activation system and hence is not likely to classify as a gene mutant in vitro.

In yet another study, gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical by membrane method. In this method, a solution of the test chemical ( 1000 µg/ 20mL agar plate) was first spread over a well-dried surface of plain 2 per cent agar. The plates were allowed to stand for about 1 hr to permit diffusion of the chemical into the agar. A sterilized sheet of cellophane membrane, cut out from dialysis tubing, was next placed on the surface of the agar and inoculated with approximately 10⁸washe cells of E. coli Sd4-73 in 0.05 ml of distilled water. The suspension was spread evenly over the membrane, and with a sufficiently dry plate, soaked rapidly into the medium. The plates were incubated at 36 C for 2 hr and the membranes were then removed carefully from the plain agar surface and transferred to the surface of a nutrient agar plate, which was incubated for at least 5 days to permit the development of mutant colonies. A second membrane treated in the same manner was placed on the surface of streptomycin agar, to detect the inhibition, if any, by the test chemical. Likewise, a third similarly treated membrane placed on the surface of streptomycin-containing minimal agar served as a test for mutation at the cystine locus. Mutagenicity for the streptomycin-dependence locus was indicated by the development of a large number of streptomycin independent colonies on the first membrane as compared with the control. Similarly, mutagenicity for the cystine locus was manifested by an increased colony count on the third membrane. The relative mutaganicity of chemical was found to be 2. The test chemical did not induce gene mutation in E. coli strain Sd4-73 by the membrane method performed an hence is not likely to be a gene mutant in vitro.

In the same study gene mutation toxicity study was also performed to determine the mutagenic nature of the test chemical by paper disc method. A culture of E. coli strain Sd4-73 was prepared in nutrient broth containing 20,g per ml streptomycin by incubation overnight at 36 C with aeration. Aeration by shaking was found to be as suitable as forced aeration. The culture was centrifuged, washed twice in distilled water to remove streptomycin (a compulsory step), and resuspended in saline to a concentration of approximately 10⁹cells per ml. The number of cells per plate was found to be critical, the yield of mutant colonies being reduced either by crowding or by insufficient population size. A 0.1 ml amount of this suspension was inoculated into 2.5 ml of soft nutrient agar (0.7% agar) on a base of 20 ml of 2 per cent nutrient agar. The use of an additional plate seeded with a tenfold lower dilution is recommended. Seeding by layering with soft agar was found to give more uniform results than spreading the suspension over the surface of the medium. After the agar had set, but without much further delay, a sterile filter paper disc of a standard diameter of 12.7 mm was placed on the surface of the medium and moistened with 0.1 ml of a solution of the test chemical. Plates of nutrient agar containing 100 g per ml of streptomycin could be seeded in parallel to give an indication of the size of the inhibition zone, if any, with the amount of the test chemical employed. Maximum expression of mutagenicity on streptomycin free test plates was usually obtained when testing chemicals at concentration levels where a narrow zone of inhibition was demarcated around the disc on streptomycin- containing control plates. Petri dishes were incubated at 36 C for at least 5 days, at which time they were examined for mutant colonies. Positive results were characterized by the appearance of several colonies around the mutagen saturated discs on nutrient agar, while parallel mutagen-free plates exhibited no growth or significantly fewer colonies. The mutant colonies were arranged around the disc in a ring, the inner and outer radii of which were determined by the amount of mutagen, toxicity, rate of diffusion, and the range of effective mutagenic concentration. The test chemical did not induce gene mutation in E. coli strain Sd4-73 by the paper disc method performed and hence is not likely to be a gene mutant in vitro.

Chromosome aberration study:

In vitro chromosomal aberration test was performed to determine the mutagenic nature of the test chemical. The study was performed using Human peripheral blood lymphocytes with and without S9 metabolic activation system. The test chemical was dissolved in distilled water and used at dose level of0, 0.33, 1.0, 3.3, 10 mM. Concurrent solvent and positive control plates were also included in the study. The test chemical did not induce chromosome aberrations in Human peripheral blood lymphocytes with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

In another study, an in vitro mammalian cell gene Mutation test was carried out according to OECD guideline 476 using Chinese hamster ovary cells to evaluate the genetic potential of the test chemical. In results, The test chemical did not induced any chromosomal aberrations at concentrations up to 150-1000 μg/plate and 50-1000 μg/plate in Chinese hamster ovary cells in the presence and absence of S9 metabolic activation system and hence it is not likely to classify as a gene mutant in vitro.

Based on the observations made, the test chemical 1H-benzimidazole (CAS no 51-17-2) did not induce chromosome aberrations with and without S9 metabolic activation system and hence is not likely to classify as gene mutant in vitro.

Based on the information observed for the test chemical, it is summarized that 1H-Benzimidazole (CAS no 51 -17 -2) is not likely to exhibit genetic toxicity. Thus, the chemical is not classified as a genetic toxicant

Justification for classification or non-classification

Based on the information observed for the test chemical, it is summarized that Benzimidazole (IUPAC name:1H-benzimidazole, CAS no 51 -17 -2) is not likely to exhibit genetic toxicity. Thus, the chemical is not classified as a genetic toxicant