3'-nitroacetophenone

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from ABITEC study report.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Principles of method if other than guideline:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 3-Nitroacetophenone according to OECD Guideline 201.

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (as cited in study report): 3-Nitroacetophenone
- Molecular formula (if other than submission substance): C8H7NO3
- Molecular weight (if other than submission substance): 165.147 g/mol
- Smiles notation (if other than submission substance): c1(cc(ccc1)[N+](=O)[O-])C(C)=O
- InChI: 1S/C8H7NO3/c1-6(10)7-3-2-4-8(5-7)9(11)12/h2-5H,1H3
- Substance type: Organic
- Physical state: Solid

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

The stock solution (100 mg/L) was prepared by dissolving light brown powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 mins. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Desmodesmus subspicatus
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Institute of botany of the ASCR
- Initial biomass concentration: 5x10(3) cells /ml

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Remarks on exposure duration:

±1 hour

Test temperature:

23±2°C

pH:

The sample at test concentration 80.0 mg/l : pH = 7.9 changed to pH = 7.7 during the test.
Control: 8.2 (changed to 7.4 during test)

Nominal and measured concentrations:

0, 2.5, 5, 10, 20, 40 and 80 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 7.5 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3
- Other:
Control solution: OECD growth medium without the sample, the coefficient of variation of average growth rate in replicates during the whole test period was 2.9%.

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7) was used as a reference substance for the study.

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

16.9 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% CL: 14.4 - 19.8

Results with reference substance (positive control):

Results with reference substance valid
- EC50: 0.69 mg/L

Validity criteria fulfilled:

not specified

Conclusions:

The median effective concentration (EC50) for the test substance 3-Nitroacetophenone in Desmodesmus subspicatus was determined to be 16.9 mg/L on the basis of effects on growth rate in a 72 hour study.

Executive summary:

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 3-Nitroacetophenone to OECD Guideline 201.

The stock solution (100 mg/L) was prepared by dissolving light brown powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 mins. Test solutions  of required concentrations  were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 2.5, 5, 10, 20, 40 and 80 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter.. ErC50 was calculated using non-linear regression by the software Prism 4.0.

The median effective concentration (EC50) for the test substance 3-Nitroacetophenone in Desmodesmus subspicatus was determined to be 16.9 mg/L on the basis of effects on growth rate in a 72 hour study.Thus, based on this value, test chemical 3-Nitroacetophenone can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.

Description of key information

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 3-Nitroacetophenone to OECD Guideline 201.

The stock solution (100 mg/L) was prepared by dissolving light brown powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 mins. Test solutions  of required concentrations  were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 2.5, 5, 10, 20, 40 and 80 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter.. ErC50 was calculated using non-linear regression by the software Prism 4.0.

The median effective concentration (EC50) for the test substance 3-Nitroacetophenone in Desmodesmus subspicatus was determined to be 16.9 mg/L on the basis of effects on growth rate in a 72 hour study.Thus, based on this value, test chemical 3-Nitroacetophenone can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

16.9 mg/L

Additional information

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 3-Nitroacetophenone to OECD Guideline 201.

The stock solution (100 mg/L) was prepared by dissolving light brown powder in OECD growth medium. The solution was kept in ultrasonic bath for 20 mins. Test solutions  of required concentrations  were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 2.5, 5, 10, 20, 40 and 80 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter.. ErC50 was calculated using non-linear regression by the software Prism 4.0.

The median effective concentration (EC50) for the test substance 3-Nitroacetophenone in Desmodesmus subspicatus was determined to be 16.9 mg/L on the basis of effects on growth rate in a 72 hour study.Thus, based on this value, test chemical 3-Nitroacetophenone can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.