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PBT assessment

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PBT assessment: overall result

Type of composition:
boundary composition of the substance
State / form:
solid: particulate/powder
Reference substance:
PBT status:
the substance is not PBT / vPvB

Classification 3,3'-(1,4-phenylenediimino)bis(4,5,6,7-tetrachloro-1H-isoindol-1-one)for effects in the environment:


The chemical3,3'-(1,4-phenylenediimino)bis(4,5,6,7-tetrachloro-1H-isoindol-1-one)(CAS no. 5590-18-1) is used as a pigment for paints, plastic, fibre and offset inks, UV inks as well as for water based ink and textile printing. It can be used in coloring raw liquor of synthetic fiber. Also used as an inert ingredient in non-food pesticide products. The aim was to assess whether the PBT criterion within Annex XIII was fulfilled for3,3'-(1,4-phenylenediimino)bis(4,5,6,7-tetrachloro-1H-isoindol-1-one). The PBT criterion was herein assessed based on experimental data in conjunction with standardized environmental fate models. Here follows a description of the PBT assessment.


Persistence assessment

The tested substance does not fulfil the P criterion within Annex XIII based on the assessment that here follows:


Biotic degradation

28-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test item. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used as a test inoculum for this study. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32 ml/l. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 72.28%. Degradation of Sodium Benzoate exceeds 63.25 % on 7 days & 69.27 % on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid. The BOD28 value of test chemical was observed to be 0.72 mgO2/mg. ThOD was calculated as 0.87 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 82.75%. Thus on the basis of result and degradation percentage observations, it is concluded that the test chemical is readily biodegradable in nature.


Thus, based on the available information, it indicates thatchemical3,3'-(1,4-phenylenediimino)bis(4,5,6,7-tetrachloro-1H-isoindol-1-one)was considered to be readily biodegradable in water and thus likely to be not persistent (not P) in nature.


Bioaccumulation assessment

The tested substancedoes not fulfil the B criterion within Annex XIII based on the assessment that here follows:


The estimated BCF value from authoritative database was determined to be ranges from 1905.0 to 1916.0, respectively. If this chemical is released into the aquatic environment, there should be a low risk for the chemical to bioaccumulate in fish and food chains.


Toxicity assessment

The tested substance does not fulfil the T criterion within Annex XIII based on the assessment that here follows:



The tested chemical is regarded to be not classified for carcinogenicity, mutagenicity and reprotoxicity, Further, there is no evidence of chronic toxicity, as identified by the classifications STOT (repeated exposure), category 1(oral, dermal, inhalation of gases/vapours, inhalation of dust/mist/fume) or category 2 (oral, dermal, inhalation of gases/vapours, inhalation of dust/mist/fume).


Aquatic organisms

As per Column 2 (Annex VIII) of the REACH regulation,testing for this end point is considered scientifically unjustified since there are mitigating factors indicating that aquatic toxicity is unlikely to occur as the substance is insoluble in water (solubility: < 0.01 mg/L at 25° C). The chemical3,3'-(1,4-phenylenediimino)bis(4,5,6,7-tetrachloro-1H-isoindol-1-one)was therefore not considered as hazardous to aquatic environments as per the criteria set out in Annex XIII.



Based on critical, independent and collective evaluation of information summarized herein, the tested compound does not fulfil the P, B and T criterion and has therefore not been classified as a PBT compound within Annex XIII.