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EC number: 943-540-0
CAS number: -
The test substance was evaluated in the
Ames/Salmonella-E.coli Liquid Pre-incubation Assay to determine its
ability to induce reverse mutations at selected histidine loci in five
tester strains of salmonella typhimurium (TA1535, TA1537, TA1538, TA98
and TA100), and at the tryptophan locus in one Escherichia coli tester
strain (WP2uvrA-), in the presence and absence of an exogenous metabolic
activation system (S9). Toxicity of the test substance was first
evaluated in a prescreen by treating duplicate culturesof strains
TA1538, TA100 and WP2uvrA- with the test article at doses of 50, 167,
500, 1670 and 5000 ug/plate in the absence of S9. Results of the
prescreen indicated OS#26589K was not toxic to tester strain WP2uvrA- at
doses of 50, 167 and 500 ug/plate. Inhibited growth (characterised by
the absence of a confluent bacterial lawn and/or the presence of pindot
colonies) or complete toxicity was observed in both Salmonella strains
at all doses evaluated, and in strain WP2uvrA- at dose =>1670ug/plate.
In addition, the test article precipitated from solution at doses =>
Based upon these findings, OS#26589K was
evaluated in triplicate cultures in all five strains of Salmonella at
doses of 0.167, 0.5, 1.67, 5, 16.7 and 50 ug/plate, and in strain
WP2uvrA- at dose of 16.7, 50, 167, 500, 1670 and 5000 ug/plate, in the
presence and absence of S9. Six dose levels were evaluated with and
without S9 in the event of unacceptable toxicity and/or insolubility at
the highest dose levels evaluated in the mutati assay. The S9 mixture
included 6% (v/v) Aroclor 1254 -induced male Sprague-Dawley rat liver
homogenate with the appropriate buffer and cofactors.The test article
again precipitated from solution =>1670 ug/plate. Inhited growth again
was observed in all Salmonella strains at doses of 5, 16.7, and/or 50
ug/plate with and/or without S9, and in tester strain WP2uvrA- at doses
of 1670 and/or 5000 ug/plate with and/or without S9. Revertant
frequencies for all doses of the test substance in all tester strains
with and without S9 approximated or were less than those observed in the
concurrent negative control cultures.
The test substance was re-evaluated in the
confirmatory assay under identical conditions, and similar results were
observed. The test article again precipitated from solution at doses
=>1670 ug/plate. Inhibited growth again was observed in all Salmonela
strains at doses of 5, 16.7 and/or 50 ug/plate with and/or without S9,
and in tester strains WP2uvrA- at doses of 1670 and/or 5000 ug/plate
with and/or without S9. Revertant frequencies for all dose levels in all
tester strains with and without S9 approximated or were less than
control value. All positive and negative control values in both assays
were within acceptable limits.
Therefore, the results for were negative in
the Ames/Salmonella-E.coli Liquid Pre-incubation Assay under the
conditions, and according to the criteria, of the test protocol.
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