Registration Dossier
Registration Dossier
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EC number: 277-749-7 | CAS number: 74181-84-3
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The study by Geerts (2010) is considered key due to the GLP guideline study wrt active sludge respiration inhibition, 3h duration. The NOEC was detemined >= 640 mg/L for EDTA-MnNa2. This would correspond to >= 707 mg/L EDTA-CuK2.
Key value for chemical safety assessment
Additional information
To investigate toxicity to microorganisms from different sources, multiple endpoints have been investigated with several EDTA salts or complexes. Respiration rate, bioluminescense, nitrification inhibition and growth of Pseudomonas have been investigated, but not all studies are equally reliable.
Geerts (2010) is preferred, supported by Hu (2003), BASF (1988) & van Ginkel (2000) all indicating that effects of the EDTA salts or complexes are not harmful to activated sludge.
To determine a PNEC for microorg the study by Hu (nitrification inhibition) should also be considered as this endpoint is generally considered more sensitive than sludge respiration. The EC50 endpoint was determined at 2.4 mmol/L for EDTA-H4 (701 mg/L) corresponding to 1032 mg/L EDTA-CuK2.
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