Reaction mass of 2-[7-isopropyl-5-methylbicyclo[2.2.2]oct-5-en-2-yl]-1,3-dioxolane and 2-[8-isopropyl-6-methylbicyclo[2.2.2]oct-5-en-2-yl]-1,3-dioxolane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study done to guideline, in compliance with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-[8-isopropyl-6-methylbicyclo[2.2.2]oct-5-en-2-yl]-1,3-dioxolane

EC Number:

272-669-9

EC Name:

2-[8-isopropyl-6-methylbicyclo[2.2.2]oct-5-en-2-yl]-1,3-dioxolane

Cas Number:

68901-32-6

Molecular formula:

C15H24O2

IUPAC Name:

2-[8-isopropyl-6-methylbicyclo[2.2.2]oct-5-en-2-yl]-1,3-dioxolane

Test material form:

other: liquid

Details on test material:

Please note that although the substance is stated as the mono-constituent substance 2-(6-methyl-8-(-1-methylethyl)bicyclo(2.2.2)oct-5-en-2(3)-yl-1,3-dioxolane in the report, analytical data on the same batch shows that the test substance is in fact the multi-constituent substance Reaction mass of 2-(7-isopropyl-5(6)-methylbicyclo[2.2.2]oct-5-en-2-yl)-1,3-dioxolane and 2-(8-isopropyl-5(6)-methylbicyclo[2.2.2]oct-5-en-2-yl)-1,3-dioxolane.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples taken from all concentrations were analysed. The actual concentrations at the start of the test ranged from 0.26 to 13 mg/l at the lowest and the highest concentration, respectively. Measured concentrations remained fairly stable during the test (75-107% of initial). The calculated average exposure concentrations corresponded to 0.24, 0.50, 1.1, 2.5, 5.4 and 12 mg/l in solutions containing 2.2, 4.6, 10, 22, 46 and 100% of the WSF prepared at 100 mg/l, respectively.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Nominal and measured concentrations:

Nominal: 2.2, 4.6, 10, 22, 46 and 100% of the water soluble fraction
Measured: 0.24, 0.50, 1.1, 2.5, 5.4 and 12 mg/l (geometric means of concentrations measured at the start and end of the test)

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.8 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% Confidence Interval

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

Under the conditions of the present study the 48h-EC50 was 2.8 mg/l based on average exposure concentrations (95% confidence interval between 2.4 and 3.7 mg/l).