1,4-dichloro-2-nitrobenzene

Endpoint summary

Description of key information

Acute oral toxicity: 

The acute oral toxicity dose (LD50) was considered based on different studies conducted on guinea pig for the test chemical 2, 5-Dichloronitrobenzene. The LD50 value is 800 mg/kg bw. The study concluded that the LD50 value is between 300-2000 mg/kg bw, for acute oral toxicity. Thus, comparing this value with the criteria of CLP regulation, the given test chemical can be classified in “Category 4” for acute oral toxicity.

Acute Inhalation Toxicity:

The acute inhalation toxicity study need not be conducted because exposure to humans via inhalation route is not likely taking into account due to the low vapour pressure of the test chemical, which is reported to be 0.005 mmHg. Thus, exposure to inhalable dust, mist and vapour of the chemical is highly unlikely. Therefore this study is considered for waiver.

Acute Dermal toxicity:

The acute dermal toxicity dose (LD50) was considered based on study conducted on rats for the given test chemical 2, 5-Dichloronitrobenzene. The study concluded that LD50 value is >2000 mg/kg bw, for acute dermal toxicity. Thus, comparing this value with the criteria of CLP regulation, the given test chemical cannot be classified for acute dermal toxicity.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Qualifier:

according to guideline

Guideline:

other: as below

Principles of method if other than guideline:

2, 5-Dichloronitrobenzene was evaluated for its toxic nature upon its acute oral administration in Guinea pigs.

GLP compliance:

not specified

Test type:

other: No data available

Species:

guinea pig

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

no data

Route of administration:

oral: gavage

Vehicle:

other: 5% gum acacia solution

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 1200, 1000, 800, 600 and 500 mg/Kg
- Amount of vehicle (if gavage): No data available
- Justification for choice of vehicle: No data available
- Lot/batch no. (if required): No data available
- Purity: No data available

MAXIMUM DOSE VOLUME APPLIED: No data available

DOSAGE PREPARATION (if unusual): No data available

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: No data available

Doses:

1200, 1000, 800, 600 and 500 mg/Kg

No. of animals per sex per dose:

14

Control animals:

not specified

Key result

Sex:

not specified

Dose descriptor:

LD50

Effect level:

800 mg/kg bw

Based on:

test mat.

Clinical signs:

2, 5 dichloronitrobenzene was fairly toxic and likewise produced some irritation in the kidneys.

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The acute oral LD50 for the test material 2, 5 dichloronitrobenzene is found to be 800mg/Kg.

Executive summary:

2, 5-Dichloronitrobenzene was evaluated for its toxic nature upon its acute oral administration in Guinea pigs.

 

The test compoundwas fairly toxic and likewise produced some irritation in the kidneys.

 

The acute oral LD50 for the test material2, 5 dichloronitrobenzene is found to be 800mg/Kg.

 

According to the publication, the test material classifies as an acute toxicant Category 4 chemical.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed

Dose descriptor:

LD50

Value:

800 mg/kg bw

Quality of whole database:

Data is secondary source (K4)

Acute toxicity: via inhalation route

Link to relevant study records

Reference

Endpoint:

acute toxicity: inhalation

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

other:

Endpoint conclusion

Quality of whole database:

Waiver

Acute toxicity: via dermal route

Link to relevant study records

Reference

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Qualifier:

according to guideline

Guideline:

other: as below

Principles of method if other than guideline:

Acute dermal toxicity of chemical 2,5-Dichloronitrobenzene was determined in rats

GLP compliance:

not specified

Test type:

other: no data

Species:

rat

Strain:

not specified

Sex:

not specified

Type of coverage:

not specified

Vehicle:

not specified

Duration of exposure:

no data

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

no data

Control animals:

not specified

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Interpretation of results:

other: not classified

Conclusions:

The LD50 value was found to be >2000 mg/kg in rats exposed to chemical 2,5-Dichloronitrobenzene in acute dermal test.

Executive summary:

Acute dermal toxiciyt test was performed to evaluate lethality of chemical 2,5-Dichloronitrobenzene.The animals were exposed by dermal route with dose concentration of 2000 mg/kg.

The animal only showed only a decrease of their spontaneous activity.2000 mg/kg did not lead to any deaths for rats.

The LD50 value was found to be >2000 mg/kg in rats exposed to chemical 2,5-Dichloronitrobenzene in acute dermal test.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Dose descriptor:

LD50

Value:

2 000 mg/kg bw

Quality of whole database:

Data is from authoritative database (K2)

Additional information

Acute oral toxicity

Various experimental studies of the test chemical were reviewed for acute oral toxicity endpoint which are summarised as below:

 

In an experimental key study from secondary source, test chemical 2, 5-Dichloronitrobenzene was evaluated for its toxic nature upon its acute oral administration in Guinea pigs. The test compound was fairly toxic and likewise produced some irritation in the kidneys. The acute oral LD50 for the test material 2, 5 dichloronitrobenzene is found to be 800mg/Kg. According to the publication, the test material classifies as an acute toxicant Category 4 chemical.

 

For the test chemical 2, 5-Dichloronitrobenzene, it was evaluated for its toxic nature upon its acute oral administration in rats. The acute oral LD50 for the test material Benzene, 1, 4-Dichloro-2-Nitro is found to be 2503 mg/Kg. According to the CLP classification, the test material does not classify as an acute oral toxicant.

Thus, based on the above summarised studies on test chemical, it can be concluded that the LD50 value is between 300-2000 mg/kg bw, for acute oral toxicity. Thus, comparing this range with the criteria of CLP regulation, the given test chemical can be classified in “Category 4” for acute oral toxicity.

 

Acute inhalation toxicity

The acute inhalation toxicity study need not be conducted because exposure to humans via inhalation route is not likely taking into account due to the low vapour pressure of the test chemical, which is reported to be 0.005 mmHg. Thus, exposure to inhalable dust, mist and vapour of the chemical is highly unlikely. Therefore this study is considered for waiver.

 

Acute dermal toxicity

Various experimental studies of the test chemical were reviewed for acute dermal toxicity endpoint which are summarised as below:

 

In an experimental key study from authoritative database, acute dermal toxicity test was performed to evaluate lethality of test chemical 2,5-Dichloronitrobenzene.The animals were exposed by dermal route with dose concentration of 2000 mg/kg. The animal only showed only a decrease of their spontaneous activity.2000 mg/kg did not lead to any deaths for rats. The LD50 value was found to be >2000 mg/kg in rats exposed to chemical 2,5-Dichloronitrobenzene in acute dermal test.

 

For the test chemical, 2, 5-Dichloronitrobenzene was evaluated for its toxic nature upon its acute dermal application in rats (secondary source). The acute dermal LD50 for the test material Benzene, 1, 4-Dichloro-2-Nitro is found to be >2000 mg/Kg. According to the CLP classification, the test material does not classify as an acute dermal toxicant.

 

The study concluded that LD50 value is >2000 mg/kg bw, for acute dermal toxicity. Thus, comparing this value with the criteria of CLP regulation, the given test chemical cannot be classified for acute dermal toxicity.

Justification for classification or non-classification

Based on the above studies for the test chemical, it can be concluded that LD50 value is between 300-2000 mg/kg bw, for acute oral toxicity and LD50 value is >2000 mg/kg bw, for acute dermal toxicity. Thus, comparing this range and value with the criteria of CLP regulation, the given test chemical can be classified in “Category 4” for acute oral toxicity and cannot be classified for acute dermal toxicity. For acute inhalation toxicity wavier was added so, not possible to classify.