Benzeneacetic acid, .alpha.-hydroxy-, ammonium salt (1:1), (.alpha.R)-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000-12-14 - 2000-12-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according to OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzeneacetic acid, .alpha.-hydroxy-, monoammonium salt, (.alpha.R)-

EC Number:

606-826-3

Cas Number:

217308-07-1

Molecular formula:

C8 H8 O3 .H3 N

IUPAC Name:

Benzeneacetic acid, .alpha.-hydroxy-, monoammonium salt, (.alpha.R)-

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): (R)-Ammoniummandelat solution (ca. 30 %), purity
- Physical state: liquid, light yellowish, Iight turbid
- Analytical purity: 29.1-31.2 % Ammoniummandelat, 70 % water
- Lot/batch No.: 30193/89
- Expiration date of the lot/batch: no limitation of storage
- Storage condition of test material: storage at the refrigerator (4-10°C)
- Water-solubility: miscible in any relation

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal activated sludge from laboratory wastewater treatment plant fed with municipal sewage and synthetic wastewater.
- Concentration of sludge: 30 mg/l

Duration of test (contact time):

14 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

20 mg/L

Based on:

DOC

Initial test substance concentration 2

Initial conc.:

118 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Test temperature: 22 +- 2°C
- pH: 7.4-7,8

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Test performed in closed vessels due to significant volatility of test substance: no
- Test performed in open system: yes


SAMPLING
- Sampling frequency: on days 0, 1, 3, 5, 7, 10, 13, 14

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: 1
- Toxicity control: 1
- Inhibition control: 1
- Adsorption control: 1

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

90 - 100

Sampling time:

14 d

Details on results:

DOC (mg/l, average values):
time BC RS IH PC AC TS
(d)
0 0.9 20.6 40.9 22.1 22.5 22.5
1 0.3 20.4 40.2 21.9 21.9 21.7
3 1.1 17.8 17.3 21.5 22.7 1.4
5 0.7 1.5 2.4 21.2 22.0 1.7
7 1.0 1.7 2.6 20.9 1.8
10 0.9 1.3 2.0 21.8 1.0
13 1.2 1.5 1.7 21.9 1.4
14 0.9 1.3 2.0 23.5 1.0


DOC decrease (%):
time RS IH PC AC TS
(d)
0 0 0 0 0 0
1 1 0 1 0 1
3 15 60 3 0 99
5 96 96 4 1 95
7 96 96 5 97
10 98 97 1 99
13 98 99 1 99
14 97 97 -6 99


BC = blank control
RS = reference substance
IH = inhibition control
PC = abiotic control
AC = adsorption control
TS = test substance

- test duration: 14 days

- adaptation phase: 1 day

- degradation phase: 2 days

- elimination of test substance by adsorption: 0-10 % after 5 days

- 10-day window met: yes

Kinetic of test substance (in %):
1 after 1 day(s)
99 after 3 day(s)
99 after 14 day(s)
Kinetic of control substance (in %):
15 after 3 day(s)
96 after 5 day(s)

BOD5 / COD results

Results with reference substance:

90-100 (% DOC) after 14 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable