Registration Dossier
Registration Dossier
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EC number: 812-739-3 | CAS number: 157357-30-7
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
EU Method C.2, OECD Guideline 202, GLP, key study, validity 2:
48h-EC50 = 18 mg/L (95% CI: 10-32 mg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 18 mg/L
Additional information
One key study is available to assess the 48h acute toxicity of the registered substance to Daphnia magna, under static conditions, according to the EU Method C.2 and the OECD Guideline 202 with GLP statement.
The nominal test substance concentrations tested were 0.32, 1.0, 3.2, 10, 32 and 100 mg/L. Additionally, a control group was tested in parallel. Since the test substance was determined to be volatile, the test was performed in 50 mL glass tubes completely filled with about 50 mL of test medium. The test tubes were made tight with glass stoppers to avoid losses of the volatile substance.
At the start and end of the test, the analytically determined concentrations of the test substance in the test media were between 93 and 142% of the nominal values. Thus, the correct dosing of the test substance was confirmed. Furthermore, it has been demonstrated that the test substance was stable during the test period of 48 hours under the conditions of the test, and the reported biological results are based on the nominal concentrations of the test substance.
All validity criteria were fulfilled but the separation factor between concentrations greatly exceeded the guideline recommendation and no effect and 100% effect concentrations were adjacent. The 48h-EC50 value was determined as a geometric mean value of the two consecutive test concentrations with 0 and 100% inhibition of mobility, at 18 mg/L (95% confidence interval: 10 -32 mg/L).
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