2-(hydroxymethylamino)ethanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Data is from High Production Volume Information System (HPVIS) EPA 1992

Justification for type of information:

Data is from High Production Volume Information System (HPVIS) EPA 1992

Qualifier:

according to guideline

Guideline:

other: EPA OPP 72-2

Principles of method if other than guideline:

48 h toxicity test of Ethanol, 2-(hydroxymethylamino) was conducted in Daphnia magna according to the EPA Series 72 of Pesticide Assessment
Guidelines.

GLP compliance:

not specified

Specific details on test material used for the study:

Name of test material: 2-[(Hydroxymethyl)amino] ethanol
Molecular formula: C3H9NO2
Molecular weight: 91.1091 g/mol
Smiles notation: CN(CCO)O
InChl : C3H9NO2/c14(6)235/h56H,23H2,1H3
Substance Type: Organic
Physical State: Liquid

Analytical monitoring:

yes

Details on sampling:

No data available

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the test substance was prepared at a concentration of 0.848 g/ml by dissolving Troysan 174 in deionized water. The stock was delivered to the diluter and mixed with well water to achieve the desired test concentrations.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:water flea
- Source: Wildlife international Ltd. Cultures
- Age at study initiation (mean and range, SD): < 24 hours
- Feeding during test: Neonate were not feeding during test but the adults were fed during the 24-hour isolation period prior to test initiation
- Food type: Daphnids in the cultures were fed a mixture of yeast, Cerophyll®, and trout chow, as well as a suspension of the freshwater green alga Selenastrum capricornutum.
- Method of breeding: Neonates were obtained for testing by transferring individual adult daphnids to dilution water 24 hours prior to test initiation.

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no data

Hardness:

148 mg/l as CaCo3 at 0 hr

Test temperature:

20±1°C

pH:

8.2-9.0

Dissolved oxygen:

9.0-9.4 mg/l

Salinity:

no data

Conductivity:

330

Nominal and measured concentrations:

Nominal concentration: 0, 13.0, 21.6, 36.0, 60.0 and 100 mg/L
Measured concentrations (mg/L):
24 hours: 0.3, 10.0, 18.2, 34.3, 60.5, 107.5 mg/L
48 hours: 0, 6.7, 9.9, 37.1, 48.1, 72.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: test chamber
- Material, size, headspace, fill volume: glass cylinders, 5 cm in diameter and 10 cm in length. The depth of the test solution in each test compartment was approximately 6 cm; leaving 4 cm headspace.
- No. of vessels per control (replicates): 2 replicates with 10 organisms each for each test concentration.
- Photoperiod: 16 hours of light & 8 hours of darkness
- Light intensity: Fluorescent tubes that emitted wavelengths similar to natural sunlight.Light intensity during the study was approximately 20 to 70 foot candles at the surface of the water.

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

13 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: no mortality, immobilization or signs of toxicity

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

27.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95 % confidence limit: 21.6-36.0 mg/l

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

54.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% confidence limits: 36-100 mg/l

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

The computer program of C.E. Stephan was used to calculate the EC50 and 95% confidence interval by probit analysis, the moving average method, or binomial probability with nonlinear interpolation. The binomial method was used to evaluate mortality at 24 and 48 hours. The No Observed Effect Concentration was determined by visually inspecting the mortality data.

Validity criteria fulfilled:

not specified

Conclusions:

The 48 hour EC50 value for daphnia exposed to Troysan 174 was 27.9 mg/L. The 95% confidence limits for the EC50 value were 21.6 and 36.0 mg/L. The 48hour No Observed Effect Concentration was 13.0 mg/L, as determined by visual observation of the toxicity data.Based on the value, 2-[hydroxy(methyl)amino]ethanol (34375-28-5) was considered to be toxic to aquatic invertebrates but as it was readily biodegradable hence, 2-[hydroxy(methyl)amino]ethanol was considered to not classified as per the CLP regulations.

Executive summary:

The test was performed in daphnia magna .Daphnia magna were exposed to Nominal concentrations (mg/L): 0, 13.0, 21.6, 36.0, 60.0 and 100 mg/L and measured concentrations (mg/L): 24 hours: 0.3, 10.0, 18.2, 34.3, 60.5, 107.5 mg/L.48 hours: 0, 6.7, 9.9, 37.1, 48.1, 72.5 mg/L of Troysan 174 and a negative (well water) control for 48h.

Daily observations of mortality and other signs of toxicity were recorded. There was no mortality, immobilization or signs of toxicity observed in the 13.0 mg/L treatment group. All daphnids in that group appeared healthy and normal throughout the test. While there were no mortalities or other signs of toxicity during the first 24 hours of exposure in the 21.6 and 36.0 mg/l treatment groups, by 48 hours mortality in those two groups was 5% and 95% respectively. At 60.0 mg/L there was 65% mortality within the first 24 hours, and 100% mortality by 48 hours. At 100 mg/L the highest concentration tested, 100% mortality occurred within 24 hours of test initiation. The 48 hour EC50 value for daphnids exposed to Troysan 174 was 27.9 mg/L. The 95% confidence limits for the EC50 value were 21.6 and 36.0 mg/L. The 48hour No Observed Effect Concentration was 13.0 mg/L, as determined by visual observation of the toxicity data.

Based on the value, 2-[hydroxy(methyl)amino]ethanol (34375-28-5) was considered to be toxic to aquatic invertebrates but as it was readily biodegradable hence, 2-[hydroxy(methyl)amino]ethanol was considered to not classified as per the CLP regulations.

Description of key information

The test was performed in daphnia magna from High Production Volume Information System (HPVIS) EPA 1992 .Daphnia magna were exposed to Nominal concentrations (mg/L): 0, 13.0, 21.6, 36.0, 60.0 and 100 mg/L and measured concentrations (mg/L): 24 hours: 0.3, 10.0, 18.2, 34.3, 60.5, 107.5 mg/L.48 hours: 0, 6.7, 9.9, 37.1, 48.1, 72.5 mg/L of Troysan 174 and a negative (well water) control for 48h.

Daily observations of mortality and other signs of toxicity were recorded. There was no mortality, immobilization or signs of toxicity observed in the 13.0 mg/L treatment group. All daphnids in that group appeared healthy and normal throughout the test. While there were no mortalities or other signs of toxicity during the first 24 hours of exposure in the 21.6 and 36.0 mg/l treatment groups, by 48 hours mortality in those two groups was 5% and 95% respectively. At 60.0 mg/L there was 65% mortality within the first 24 hours, and 100% mortality by 48 hours. At 100 mg/L the highest concentration tested, 100% mortality occurred within 24 hours of test initiation. The 48 hour EC50 value for daphnids exposed to Troysan 174 was 27.9 mg/L. The 95% confidence limits for the EC50 value were 21.6 and 36.0 mg/L. The 48hour No Observed Effect Concentration was 13.0 mg/L, as determined by visual observation of the toxicity data. Based on the value, 2-[hydroxy(methyl)amino]ethanol (34375-28-5) was considered to be toxic to aquatic invertebrates but as it was readily biodegradable hence, 2-[hydroxy(methyl)amino]ethanol was considered to not classified as per the CLP regulations.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

27.9 mg/L

Additional information

Short term toxicity to aquatic invertebrates 2-[hydroxy(methyl)amino]ethanol (34375-28-5) was summaries with experimental data for target and experimental study of functional read across are summaries as follows:

The test was performed in daphnia magna from High Production Volume Information System (HPVIS) EPA 1992 .Daphnia magna were exposed to Nominal concentrations (mg/L): 0, 13.0, 21.6, 36.0, 60.0 and 100 mg/L and measured concentrations (mg/L): 24 hours: 0.3, 10.0, 18.2, 34.3, 60.5, 107.5 mg/L.48 hours: 0, 6.7, 9.9, 37.1, 48.1, 72.5 mg/L of Troysan 174 and a negative (well water) control for 48h.

Daily observations of mortality and other signs of toxicity were recorded. There was no mortality, immobilization or signs of toxicity observed in the 13.0 mg/L treatment group. All daphnids in that group appeared healthy and normal throughout the test. While there were no mortalities or other signs of toxicity during the first 24 hours of exposure in the 21.6 and 36.0 mg/l treatment groups, by 48 hours mortality in those two groups was 5% and 95% respectively. At 60.0 mg/L there was 65% mortality within the first 24 hours, and 100% mortality by 48 hours. At 100 mg/L the highest concentration tested, 100% mortality occurred within 24 hours of test initiation. The 48 hour EC50 value for daphnids exposed to Troysan 174 was 27.9 mg/L. The 95% confidence limits for the EC50 value were 21.6 and 36.0 mg/L. The 48hour No Observed Effect Concentration was 13.0 mg/L, as determined by visual observation of the toxicity data. Based on the value, 2-[hydroxy(methyl)amino]ethanol (34375-28-5) was considered to be toxic to aquatic invertebrates but as it was readily biodegradable hence, 2-[hydroxy(methyl)amino]ethanol was considered to not classified as per the CLP regulations.

Another study from Ecotox database 2017 was performed in Daphnia magna for 48 hrs with <24 hrs age. 5 daphnia magna used in fresh water media. After the experiment, the EC 50 value for 2-[hydroxy(methyl)amino]ethanol (34375-28-5) was determined to be 25.2 mg/l on the basis of intoxication. Based on the value, 2-[hydroxy(methyl)amino]ethanol (34375-28-5) was considered to be toxic to aquatic invertebrates but as it was readily biodegradable hence, 2-[hydroxy(methyl)amino]ethanol was considered to not classified as per the CLP regulations.

For functionally similar read across 1,3-Propanediol (504-63-2) study was performed accordance with the DIN 38412, Part II (Daphnia short-time test) (German Institute of Standardization, 1982) from Water. Res. Vol. 23, No.4, pp. 495--499, 1989. The test was performed in 6-24 h old daphnids were placed in each test and control vessel, i.e: 20 animals per concentration step. The test was performed in 50 ml beaker with ground-glass stoppers each with 20 ml useful capacity. After a test period of 24 h and 48 h, the number of animals in the control and test solutions that could still swim was counted. After the experiment, the EC 50 value for 1,3-Propanediol (504-63-2) was determined to be 7417 mg/l in concentration range from 6430-8555 mg/l. Based on the result, the 1,3-Propanediol (504-63-2)was considered to be non-toxic t aquatic invertebrates and can be considered to be not classified as per the CLP regulations.

Based on the experimental studies of target and study of functional read across, it was considered that 2-[hydroxy(methyl)amino]ethanol (34375-28-5) was non-toxic to aquatic invertebrates and can be considered to be not classified as per the CLP regulations.