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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Study period:
1991
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Documentation insufficient for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1991

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Hexane-2,5-dione
EC Number:
203-738-3
EC Name:
Hexane-2,5-dione
Cas Number:
110-13-4
Molecular formula:
C6H10O2
IUPAC Name:
hexane-2,5-dione
Test material form:
not specified

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals or test system and environmental conditions:
75-90 days old female Wistar rats
170-200 g body weight
12 hour light/dark cycle

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: 120 mM NaCl, 10 mM K-Phosphate
Details on exposure:
1 mL/kg bw intraperitoneal in vehicle (120 mM NaCl, 10 mM K-Phosphate, pH = 7.2)
Doses:
200, 400, 800 mg/kg bw
No. of animals per sex per dose:
15 -17 female rats per group
Control animals:
other: vehicle
Details on study design:
nociception measure at 10, 30, 60 an 90 minutes after dosing
a tail-flick-apparatus was used for the experiment (tail-flick latencies)
Statistics:
ANOVA

Results and discussion

Effect levelsopen allclose all
Sex:
female
Dose descriptor:
other: nociception
Effect level:
ca. 200 mg/kg bw
Based on:
test mat.
Sex:
female
Dose descriptor:
other: analgesia
Effect level:
ca. 400 mg/kg bw
Based on:
test mat.
Mortality:
no
Clinical signs:
antinociception, analgesia
Body weight:
no data
Gross pathology:
no data
Other findings:
none

Applicant's summary and conclusion

Conclusions:
All doses caused antinociception, the high and mid dose at all time points.
800 mg/kg bw: analgesia from 10-60 min
400 mg/kg bw: analgesia from 30-60 min
200 mg/kg bw: antinociception only at 60 min