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Registration Dossier
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EC number: 203-738-3 | CAS number: 110-13-4
Repeated dose toxicity: other routes
Administrative data
- Endpoint:
- short-term repeated dose toxicity: other route
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Unsuitable test system
Data source
Reference
- Reference Type:
- publication
- Title:
- Long-Term Treatment with 2,5-Hexanedione Has No Effect on the Specific Activity of Some Brain and Liver Glycolytic Enzymes of Adult Rats
- Author:
- M.E. Pereira, A.M. Bordignon, C. Bürger, C.I. Huang, J.B.T. Rocha
- Year:
- 1 991
- Bibliographic source:
- Brazilian J. Med Biol. Res. 1991, 24, 735-740
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- Hexane-2,5-dione
- EC Number:
- 203-738-3
- EC Name:
- Hexane-2,5-dione
- Cas Number:
- 110-13-4
- Molecular formula:
- C6H10O2
- IUPAC Name:
- hexane-2,5-dione
- Test material form:
- not specified
- Details on test material:
- no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- female Wistar rats, 40-45 days old, 150-170 g bw, 12 h light/12 h dark cycle
Administration / exposure
- Route of administration:
- subcutaneous
- Vehicle:
- other: 100 mM NaCl, 10 mM K-Phosphate buffer, pH = 7
- Details on exposure:
- 200 mg/ kg bw 2,5- Hexanedione
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 40 days
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
200 mg/ kg bw
- No. of animals per sex per dose:
- one dose group
- Control animals:
- yes
- Details on study design:
- 40 days treatment, examination of brain and liver after treatment.
Enzymes: Lactic dehydrogenase, Enolase, Malate dehydrogenase, Liver glycogene level
Examinations
- Observations and examinations performed and frequency:
- Body weight, food and water intake were measured every 6 days.
- Sacrifice and pathology:
- Decapitation, removal and analysis of brain and liver.
- Statistics:
- no data
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Mortality:
- no mortality observed
- Body weight and weight changes:
- effects observed, treatment-related
- Description (incidence and severity):
- reduced body weight gain
- Food consumption and compound intake (if feeding study):
- no effects observed
- Water consumption and compound intake (if drinking water study):
- effects observed, treatment-related
- Description (incidence and severity):
- increase
Effect levels
- Dose descriptor:
- other: enzyme activity
- Effect level:
- ca. 200 mg/kg bw/day
- Based on:
- test mat.
- Sex:
- female
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
- Conclusions:
- Reduced weight gain, increased water intake, no changes in enzyme activity in brain and liver.
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