1-chloro-3-nitrobenzene

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: OECD proposal (1979)

Principles of method if other than guideline:

96 hr short term toxicity study was conducted to test the effects of 1Chloro-3-nitrobenzene on fresh water green algae.

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data available

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: 3.6 mg/l

Vehicle:

yes

Details on test solutions:

DMSO:Dimethyl sulfoxide
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
Stock solutions of the compounds were prepared in dimethylsulfoxide DMSO

Test organisms (species):

Scenedesmus pannonicus

Details on test organisms:

TEST ORGANISM
- Common name: Green algae

Test type:

static

Water media type:

freshwater

Total exposure duration:

14 d

Post exposure observation period:

No data available

Hardness:

No data available

Test temperature:

No data available

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Nominal and measured concentrations:

No data available

Details on test conditions:

- Renewal rate of test solution (frequency/flow rate): Daily

Reference substance (positive control):

not specified

Duration:

14 d

Dose descriptor:

EC50

Effect conc.:

1.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Validity criteria fulfilled:

not specified

Conclusions:

Based on the effective minimum inhibitory concentration the EC50 was determined to be 3.06mg/l.
Classification may not be done as the study is chronic.

Executive summary:

Chronic toxicity study was carried out for 14 days to test the effects of the test substance on fresh water green algaeScenedesmus pannonicus.

Semistatic conditions were maintained and the renewal of test solution was done daily thereforetheactual LC50 values will be about 85% of the actual concentration used .

Based on the effective minimum inhibitory concentration the EC50 was determined to be 3.06mg/l.

Description of key information

Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical the EC50 was determine to be 15.1 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:

15.1 mg/L

Additional information

To evaluate toxicity of aquatic algae, various experimental studies were performed.Based on the experimental data aquired from various sources following is the discription:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100.0 g/L was prepared by dissolving light brown solid sample in DMSO. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.Various concentration were used.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.The median effective concentration (EC50) for the test substance 1-chloro-3-nitrobenzene, in algae was determined to be 15.1 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous/ non-hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

The above study was further supported by Chronic toxicity study was carried out for 14 days to test the effects of the test substance on fresh water green algae Scenedesmus pannonicus.

Semistatic conditions were maintained and the renewal of test solution was done daily thereforetheactual LC50 values will be about 85% of the actual concentration used .

Based on the effective minimum inhibitory concentration the EC50 was determined to be 3.06 mg/l.

Both the abpve studies were again supported by 96 hr short term toxicity studies which have been carried out to evaluate the effects of 1-Chloro-3-nitrobenzene on Green algaeChlorella pyrenoidosa

Based on the changes in growth the EC50 was determined to be 1.9 mg/l.

Even though varied results for toxicity of aquatic algae were available but, based on the maximum notifiers in ECHA and the effect concentration from first experimental study report,we can consider that the test chemical is toxic and can be classified as aquatic chronic 3.