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EC number: 204-496-1 | CAS number: 121-73-3
- Life Cycle description
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- Endpoint summary
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: OECD proposal (1979)
- Principles of method if other than guideline:
- 96 hr short term toxicity study was conducted to test the effects of 1Chloro-3-nitrobenzene on fresh water green algae.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data available - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 3.6 mg/l
- Vehicle:
- yes
- Details on test solutions:
- DMSO:Dimethyl sulfoxide
- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
Stock solutions of the compounds were prepared in dimethylsulfoxide DMSO - Test organisms (species):
- Scenedesmus pannonicus
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 14 d
- Post exposure observation period:
- No data available
- Hardness:
- No data available
- Test temperature:
- No data available
- pH:
- No data available
- Dissolved oxygen:
- No data available
- Salinity:
- No data available
- Nominal and measured concentrations:
- No data available
- Details on test conditions:
- - Renewal rate of test solution (frequency/flow rate): Daily
- Reference substance (positive control):
- not specified
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- No data available
- Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- No data available
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the effective minimum inhibitory concentration the EC50 was determined to be 3.06mg/l.
Classification may not be done as the study is chronic. - Executive summary:
Chronic toxicity study was carried out for 14 days to test the effects of the test substance on fresh water green algaeScenedesmus pannonicus.
Semistatic conditions were maintained and the renewal of test solution was done daily thereforetheactual LC50 values will be about 85% of the actual concentration used .
Based on the effective minimum inhibitory concentration the EC50 was determined to be 3.06mg/l.
Reference
Description of key information
Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical the EC50 was determine to be 15.1 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 15.1 mg/L
Additional information
To evaluate toxicity of aquatic algae, various experimental studies were performed.Based on the experimental data aquired from various sources following is the discription:
Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100.0 g/L was prepared by dissolving light brown solid sample in DMSO. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.Various concentration were used.With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.The median effective concentration (EC50) for the test substance 1-chloro-3-nitrobenzene, in algae was determined to be 15.1 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous/ non-hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.
The above study was further supported by Chronic toxicity study was carried out for 14 days to test the effects of the test substance on fresh water green algae Scenedesmus pannonicus.
Semistatic conditions were maintained and the renewal of test solution was done daily thereforetheactual LC50 values will be about 85% of the actual concentration used .
Based on the effective minimum inhibitory concentration the EC50 was determined to be 3.06 mg/l.
Both the abpve studies were again supported by 96 hr short term toxicity studies which have been carried out to evaluate the effects of 1-Chloro-3-nitrobenzene on Green algaeChlorella pyrenoidosa
Based on the changes in growth the EC50 was determined to be 1.9 mg/l.
Even though varied results for toxicity of aquatic algae were available but, based on the maximum notifiers in ECHA and the effect concentration from first experimental study report,we can consider that the test chemical is toxic and can be classified as aquatic chronic 3.
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