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Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

in vitro gene mutation study in bacteria
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed journal

Data source

Reference Type:
Mutagenicity of mono-nitrobenzene derivatives in the Ames test and rec assay
Makoto Shimizu and Eiji Yan
Bibliographic source:
Mutation Research, 170 (1986) 11-22

Materials and methods

Test guideline
according to guideline
other: as mentioned below
Principles of method if other than guideline:
No significant lethal effect on the tester strain was observed in the presence or absence of S9 mix at any of the dose levels tested.
GLP compliance:
Type of assay:
bacterial gene mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
- Name of test material (as cited in study report): 1-Chloro-3-nitrobenzene
- Molecular weight (if other than submission substance): 157.56
- Smiles notation (if other than submission substance): c1(cc(ccc1)Cl)[N+](=O)[O-]
- InChl (if other than submission substance): 1S/C6H4ClNO2/c7-5-2-1-3-6(4-5)8(9)10/h1-4H
- Substance type: Organic
- Physical state: Solid


Target gene:
No data available
Species / strain
Species / strain / cell type:
S. typhimurium, other: Salmonella typhimurium TA98 and TA100.
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
The S9 fraction was prepared from the liver of PCB-treated male rats. The S9 mix (0.5 ml) contained 2 Micromoles NADPH, 2.5 micro moles G6P, 4 micro moles MgCl2, and 150 microliter S9 fraction containing 2.5 mg protein
Test concentrations with justification for top dose:
Vehicle / solvent:
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
- Preincubation period:
20 min at 37 deg celcius
Evaluation criteria:
Dose response curves
No data available

Results and discussion

Test results
Species / strain:
S. typhimurium, other: S. typhimurium strain TA98 and TA100.
Metabolic activation:
with and without
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
No data available
Remarks on result:
other: No mutagenic potential

Applicant's summary and conclusion

Interpretation of results (migrated information):
negative Negative with and without

No significant lethal effect on the tester strain was observed in the presence or absence of S9 mix at any of the dose levels tested. No of His revertants in TA 98-(Without S9 mix) is 24,and in TA100 is 80. With activation-Without norharman-40, With norharman-69 In the presence of norharman, however, test chemical and exhibited mutagenicity to S.typhimurium TA98 in the presence of S9 mix indicating that norharman was effective only for the frame-shift mutagen.
Executive summary:

Study was conducted to test the effects of the given test chemical on salmonella on two strains TA 98 an TA100 using DMSO as a vehicle. Study is carried out based on Ames test with some modifications. Preincubation is done at 37 deg celcius for 20min.

No significant lethal effect on the tester strain TA 98 and TA 100 was observed in the presence or absence of S9 mix at any of the dose levels tested.