N-[2-[(2-chloro-4,6-dinitrophenyl)azo]-5-(diallylamino)-4-methoxyphenyl]acetamide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

15 min of sonication of diluted solution from a stock solution of 0.1 g/L

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

261 mg/L CaCO3

Test temperature:

20 °C

pH:

8.3

Dissolved oxygen:

97%

Nominal and measured concentrations:

Nominal: 0, 0.9, 1.9, 4.3, 9.4, 21, 45 and 100 mg/L

Details on test conditions:

10 Daphnids for each concentration in duplicate

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 47 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

ca. 4.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

47 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Replicate	Replicate	Concentration mg/l	24h		48h		% immobility
1	2	blank	0	0	0	0	0
29	30	0.9	0	0	0	0	0
31	32	1.9	0	0	0	0	0
33	34	4.3	0	0	0	0	0
35	36	9.4	0	2	4	5	45
37	38	21	0	0	3	2	25
39	40	45	0	1	4	3	35
		100	0	0	1	2	20

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the 48 h EC0, 50 and 100 for Daphnia magna were determined to be 4.3, >100 and >100 mg/L (nominal), respectively, equivalent to 2.0, >47 and >47 mg a.i./L, respectively.

Executive summary:

A study was conducted to determine the toxicity of the test substance (46.9% purity) to aquatic invertebrates (Daphnia magna) according to OECD Guideline 202. Groups of 10 Daphnia were exposed for 48 h under static conditions at nominal concentrations of 0, 0.9, 1.9, 4.3, 9.4, 21, 45 and 100 mg/L, equivalent to 0, 0.4, 0.9, 2.0, 4.4, 10, 21 and 47 mg a.i./L. The swimming mobility was assessed after 24 and 48 h. No analytical monitoring of test concentrations was conducted. Test material was observed to fall out of solution as concentrations increased, due to the hardness of the test medium. Under the study conditions, the 48 h EC0, 50 and 100 were determined to be 4.3, >100 and >100 mg/L (nominal), respectively, equivalent to 2.0, >47 and >47 mg a.i./L, respectively (Böttcher, 1997).

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

08-05-1990 to 22-06-1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

yes

Remarks:

Cremophor

Details on test solutions:

The test substance was dissolved in cremophore and the diluted with deionised water. After the dispersion, the solution is stirred mechanically to produce the stock solution. The stock solution contains 500 mg/L of the test substance.

A series of dilution was prepared with the diltuion water. 10 mL of each corresponding dilution was added per test bottle.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test strain of Daphnia magna Straus was obtained from the Institute National de Recherche Chimique Appliquee, France. The strain is being cultured since 1978 in the Laboratory for Environmental Analysis and Ecology, BASF AG Ludwigshafen.

Age of the animals: 2-24 h

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.82 mmol/L

Test temperature:

19-21°C

pH:

7.96-8.2

Dissolved oxygen:

8.01-8.94

Conductivity:

600-700 µSiemens/cm

Nominal and measured concentrations:

- Nominal: 0, 0.07, 0.2, 0.4, 0.8, 1.6, 3.1, 6.2, 13, 25, 50 and 100 mg test substance/L

Details on test conditions:

TEST SYSTEM
- Aeration: The test water is aerated with oxygen-free air until oxygen saturation and then left to stand for at least 24 h.
- No. of organisms per vessel: five
- No. of vessels per concentration (replicates): four
- No. of vessels per control (replicates): four
- No. of vessels per vehicle control (replicates): four

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionised water
- Ca/mg ratio: 4:1
- Na/K ratio: 10:1
- Conductivity: 600-700 µSiemens/cm

OTHER TEST CONDITIONS
- Photoperiod: 16 h light: 8 h dark

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

1.56 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.6 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

14.36 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.7 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

40 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the 48 h EC0, 50, and 100 (in terms of swimming mobility) of the test substance were determined to be 1.56, 14.36 and 100 mg test substance/L (nominal), respectively.

Executive summary:

A study was conducted to determine the toxicity of the test substance (40% purity) to aquatic invertebrates (Daphnia magna Straus) according to Method C.2. The Daphnia were exposed for 48 h at nominal concentrations up to 100 mg/L under static conditions. The swimming mobility was assessed after 0, 3, 24 and 48 h. No analytical monitoring of test concentrations was conducted. The average water pH was 7.78 and the water hardness 2.83 mmol/L. Under the study conditions, the nominal 48 h EC0, 50 and 100 based on mobility were determined to be 1.6, 14 and 100 mg test substance/L (nominal), equivalent to 0.6, 5.7 and 40 mg a.i./L (Jatzek, 1990).

Description of key information

The structural analogue substance Disperse Blue 79:1 Br was used in two tests to determine the short-term toxicity to aquatic invertebrates. The test conducted with the highest substance purity was selected as key study: 48-h EC50 to Daphnia magna > 100 mg/L, nominal (OECD 202). The EC50 is far above the water solubility limit of both source and target chemical.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Remarks:

48 h

Effect concentration:

> 100 mg/L