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Registration Dossier
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EC number: 231-992-5 | CAS number: 7783-35-9
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- calculation (if not (Q)SAR)
- Remarks:
- Migrated phrase: estimated by calculation
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Influence of glyphosate and its formulation (Roundup) on the toxicity and bioavailability of metals to Ceriodaphnia dubia
- Author:
- Martin T.K. Tsui, Wen-Xiong Wang , L.M. Chu ,
- Year:
- 2 005
- Bibliographic source:
- Environmental Pollution 138 (2005) 59-68
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as per below mentioned
- Principles of method if other than guideline:
- Short term toxicity was performed on Ceriodaphnia dubia (Neonate) for 48 hrs to calculate LC 100 value.
- GLP compliance:
- no
Test material
- Reference substance name:
- Mercury sulphate
- EC Number:
- 231-992-5
- EC Name:
- Mercury sulphate
- Cas Number:
- 7783-35-9
- Molecular formula:
- H2O4S.Hg
- IUPAC Name:
- mercury sulfate
- Details on test material:
- - Name of test material: Mercury sulphate
- Molecular formula: H2O4S.Hg
- Molecular weight : 298.67 g/mol
- Smiles notation: [O-]S(=O)(=O)[O-].[Hg+2]
- InChl : InChI=1S/Hg.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2
- Substance type: Solid
- Physical state: Inorganic
- Impurities (identity and concentrations): No data
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No data available
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- No data available
Test solutions
- Vehicle:
- not specified
- Details on test solutions:
- No data available
Test organisms
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- Details on test organisms
TEST ORGANISM
- Common name: Water flea
- Strain: No data available
- Source: Aquatic Research Organisms (Hampton, NH, USA)
- Age at study initiation (mean and range, SD): Neonate (<24 hrs)
- Weight at study initiation (mean and range, SD): No data available
- Length at study initiation (length definition, mean, range and SD): No data available
- Valve height at study initiation, for shell deposition study (mean and range, SD): No data available
- Peripheral shell growth removed prior to test initiation: No data available
- Method of breeding: No data available
- Feeding during test: not fed during the test
- Food type: Selenastrum capricornutum and yeastecerophyle
trout chow
- Amount: No data available
- Frequency: No data available
ACCLIMATION
- Acclimation period: No data available
- Acclimation conditions (same as test or not): No data available
- Type and amount of food:
- Feeding frequency: No data available
- Health during acclimation (any mortality observed): No data available
QUARANTINE (wild caught)
- Duration: No data available
- Health/mortality: No data available
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No data available
Test conditions
- Hardness:
- No data available
- Test temperature:
- 25°C
- pH:
- No data available
- Dissolved oxygen:
- No data available
- Salinity:
- No data available
- Nominal and measured concentrations:
- Σ0.5, Σ1.0, Σ1.5, Σ2.0 and Σ2.5 toxic units
- Details on test conditions:
- Details on test conditions
TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 ml
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: five organisms were stocked in each replicate (i.e. 10 mL per individual).
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 1 vessels
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available
OTHER TEST CONDITIONS
- Adjustment of pH: No data available
- Photoperiod: No data available
- Light intensity: No data available
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : No data available
TEST CONCENTRATIONS
- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study: No data available
- Test concentrations: Σ0.5, Σ1.0, Σ1.5, Σ2.0 and Σ2.5 toxic units
- Results used to determine the conditions for the definitive study: No data available - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 0.028 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: Σ0.5, Σ1.0, Σ1.5, Σ2.0 and Σ2.5 toxic units
- Details on results:
- No data available
- Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- No data available
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The LC 100 value for Ceriodaphnia dubia was found to be 20 µg L-1 after 48 hrs of exposure with Sulfuric acid, Mercury(2+) salt(1:1) (7783-35-9)
- Executive summary:
Acute toxicity test was performed on Ceriodaphnia dubia for 48 hrs using neonates. The experiment was performed in 100 mL beaker having 50 ml test solution. Experiment design was static with six concentrations having 4 replicates in which five organisms were stocked in each replicates. The tested concentrations in the binary mixtures were
Σ 0.5, Σ 1.0, Σ 1.5, Σ 2.0 and Σ 2.5 toxic units. Mortality was the effect measured in experiment and number of animals was counted at 24 and 48 hrs.
Therefore, on the basis of above experiment the LC 100 value for Ceriodaphnia dubia was found to be 20 µg L-1after 48 hrs of exposure with Sulfuric acid, Mercury(2+) salt(1:1) (7783-35-9).
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