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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

short-term toxicity to aquatic invertebrates
Type of information:
calculation (if not (Q)SAR)
Migrated phrase: estimated by calculation
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from peer reviewed journal

Data source

Reference Type:
Influence of glyphosate and its formulation (Roundup) on the toxicity and bioavailability of metals to Ceriodaphnia dubia
Martin T.K. Tsui, Wen-Xiong Wang , L.M. Chu ,
Bibliographic source:
Environmental Pollution 138 (2005) 59-68

Materials and methods

Test guideline
according to guideline
other: as per below mentioned
Principles of method if other than guideline:
Short term toxicity was performed on Ceriodaphnia dubia (Neonate) for 48 hrs to calculate LC 100 value.
GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
Mercury sulphate
EC Number:
EC Name:
Mercury sulphate
Cas Number:
Molecular formula:
mercury sulfate
Details on test material:
- Name of test material: Mercury sulphate
- Molecular formula: H2O4S.Hg
- Molecular weight : 298.67 g/mol
- Smiles notation: [O-]S(=O)(=O)[O-].[Hg+2]
- InChl : InChI=1S/Hg.H2O4S/c;1-5(2,3)4/h;(H2,1,2,3,4)/q+2;/p-2
- Substance type: Solid
- Physical state: Inorganic
- Impurities (identity and concentrations): No data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No data available

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
No data available

Test solutions

not specified
Details on test solutions:
No data available

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
Details on test organisms
- Common name: Water flea
- Strain: No data available
- Source: Aquatic Research Organisms (Hampton, NH, USA)
- Age at study initiation (mean and range, SD): Neonate (<24 hrs)
- Weight at study initiation (mean and range, SD): No data available
- Length at study initiation (length definition, mean, range and SD): No data available
- Valve height at study initiation, for shell deposition study (mean and range, SD): No data available
- Peripheral shell growth removed prior to test initiation: No data available
- Method of breeding: No data available
- Feeding during test: not fed during the test
- Food type: Selenastrum capricornutum and yeastecerophyle
trout chow
- Amount: No data available
- Frequency: No data available

- Acclimation period: No data available
- Acclimation conditions (same as test or not): No data available
- Type and amount of food:
- Feeding frequency: No data available
- Health during acclimation (any mortality observed): No data available

QUARANTINE (wild caught)
- Duration: No data available
- Health/mortality: No data available

Study design

Test type:
Water media type:
Total exposure duration:
48 h
Post exposure observation period:
No data available

Test conditions

No data available
Test temperature:
No data available
Dissolved oxygen:
No data available
No data available
Nominal and measured concentrations:
Σ0.5, Σ1.0, Σ1.5, Σ2.0 and Σ2.5 toxic units
Details on test conditions:
Details on test conditions
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 100 ml
- Aeration: No data available
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): No data available
- No. of organisms per vessel: five organisms were stocked in each replicate (i.e. 10 mL per individual).
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 1 vessels
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available

- Source/preparation of dilution water: No data available
- Total organic carbon: No data available
- Particulate matter: No data available
- Metals: No data available
- Pesticides: No data available
- Chlorine: No data available
- Alkalinity: No data available
- Ca/mg ratio: No data available
- Conductivity: No data available
- Culture medium different from test medium: No data available
- Intervals of water quality measurement: No data available

- Adjustment of pH: No data available
- Photoperiod: No data available
- Light intensity: No data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : No data available

- Spacing factor for test concentrations: No data available
- Justification for using less concentrations than requested by guideline: No data available
- Range finding study: No data available
- Test concentrations: Σ0.5, Σ1.0, Σ1.5, Σ2.0 and Σ2.5 toxic units
- Results used to determine the conditions for the definitive study: No data available
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
48 h
Dose descriptor:
Effect conc.:
0.028 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Remarks on result:
other: Σ0.5, Σ1.0, Σ1.5, Σ2.0 and Σ2.5 toxic units
Details on results:
No data available
Results with reference substance (positive control):
No data available
Reported statistics and error estimates:
No data available

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
The LC 100 value for Ceriodaphnia dubia was found to be 20 µg L-1 after 48 hrs of exposure with Sulfuric acid, Mercury(2+) salt(1:1) (7783-35-9)
Executive summary:

Acute toxicity test was performed on Ceriodaphnia dubia for 48 hrs using neonates. The experiment was performed in 100 mL beaker having 50 ml test solution. Experiment design was static with six concentrations having 4 replicates in which five organisms were stocked in each replicates. The tested concentrations in the binary mixtures were

Σ 0.5, Σ 1.0, Σ 1.5, Σ 2.0 and Σ 2.5 toxic units. Mortality was the effect measured in experiment and number of animals was counted at 24 and 48 hrs.

Therefore, on the basis of above experiment the LC 100 value for Ceriodaphnia dubia was found to be 20 µg L-1after 48 hrs of exposure with Sulfuric acid, Mercury(2+) salt(1:1) (7783-35-9).