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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23 Jan - 24 Feb 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
- Concentrations:
- all concentrations at t = 0 h from fresh and t = 24 h from aged solutions
- from control, 0.299, 0.657 and 1.45 mg/L at = 24 h fresh, t = 48 h fresh, t = 72 h fresh and t = 96 h aged
- Sampling method: 500 μL samples were taken and stabilized with 500 μL methanol.
- Sample storage conditions before analysis: All samples were stored deep frozen.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test item for preparing the stock solution was weighed and transferred to a volumetric flask. Test medium was added up to the bench mark and the stock solution was homogenised by shaking and treated with 30 minutes of ultrasonication. The test solutions were prepared by diluting the stock solution with test medium.
Test solutions from the concentration 1.45 mg/L and higher were slightly mikly.
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout
- Source: Forellenzucht Peter Störk, D-88348 Bad Saulgau, Germany

ACCLIMATION
- Acclimation period: > 12 days
- Acclimation conditions: Fish were held under testing conditions in 300 L containers with continuous renewal of water (5 - 10 % per day) and permanent aeration of water for the entire pre-experimental period.
- Feeding frequency during acclimation: granular rearing food with approx. 2 % of their body-weight
- Health during acclimation (any mortality observed): no mortalities observed

FEEDING DURING TEST
none
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
10° dH corresponding to 178 mg CaCO3/L
Test temperature:
13.8 – 14.4 °C
pH:
7.38 – 8.37
Nominal and measured concentrations:
Nominal: 0.0, 0.299, 0.657, 1.45, 3.18 and 7.00 mg/L
Measured concentrations in fresh test solutions was between 86 % and 103 % of nominal
Measured concentrations in aged test solutions was between 84 % and 104 % of nominal
Details on test conditions:
TEST SYSTEM
- Test vessel: 25 L glass aquaria filled with 15 L of test solution
- Aeration: continuous aeration of the test tanks with a membrane pump using a Pasteur pipette
- Renewal rate of test solution (frequency/flow rate): each 24 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.63 g fish/L

TEST MEDIUM / WATER PARAMETERS
Dechlorinated drinking water and deionised water with a conductivity of 403 μS/cm.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours daily
- Light intensity: not stated

EFFECT PARAMETERS MEASURED: Mortality and visible abnormalities as: loss of equilibrium, swimming behaviour, respiratory function, pigmentation and all other observed events were recorded after 0, 4, 24, 48, 72 and 96 hours.

TEST CONCENTRATIONS
- Range finding study: no data
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.976 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
- Behavioural abnormalities:
- Observations on body length and weight: average weight of the test organisms was 0.94 ± 0.46 g; the average length was 45 ± 5 mm
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: none
- Effect concentrations exceeding solubility of substance in test medium: none
Results with reference substance (positive control):
not available
Sublethal observations / clinical signs:

Table: % mortality of fish in test solutions:

  0 [mg/L] 0.299 mg/L] 0.657 mg/L] 1.45 mg/L] 3.18 mg/L] 7 mg/L]
Time [h] Mortality [%]
4 0 0 0 0 0 0
24 0 0 0 0 100 100
48 0 0 0 14 100 100
72 0 0 0 43 100 100
96 0 0 14 86 100 100

Description of key information

96h LC50 (Oncorhynchus mykiss, mortality) = 0.976 mg/L (nominal concentration, OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.976 mg/L

Additional information

The short-term toxicity to fish study was performed according to OECD 203 guideline under GLP conditions (Unger, 2017) In a 96 hours acute toxicity study, rainbow trouts (O. mykiss) were exposed to C14-16AS Na at nominal concentrations of 0 (control), 0.299, 0.657, 1.45, 3.18 and 7.0 mg/L. Seven organisms per test concentration were used. The 96h LC50 was 0.976 mg/L (nominal concentration). The analytical verified recovery of the test substance was above > 80%, which allows to report the result as a nominal value.