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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The 48-hour EL50 in Daphnia magna was reported as 0.41 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.41 mg/L

Additional information

In a GLP study based on OECD Guideline 203 and EU Method C.1, the acute toxicity of the test material to the water flea (Daphnia magna) was determined in a 48-h static toxicity test conducted in sealed vessels without renewal of the test media. Groups of 20 Daphnids, aged <24 h, were exposed to water accommodated fractions (WAFs) of the test material in freshwater at nominal loading rates of 0.01, 0.03, 0.1, 0.3, 1.0, 3.0 and 10.0 mg/L. Mortality and immobility of the daphnids (i.e. the number not seen to swim when observed for 15 seconds) was assessed after 24 and 48 h.

The 48-h EL50 (50 % immobilisation) was calculated to be 0.41 mg/L and the 24-h EL50 is considerd greater than 10 mg/L (the highest nominal loading rate tested). Mortality did not exceed 10 % at up to 0.1 mg/L, and this is considered the 48-h NOELR (no-observed-effect loading rate).