Reaction mass of sodium 4-(4-acetamidophenylamino)-1-amino-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate and hydrogen 4-(4-acetamidophenylamino)-1-amino-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD5

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

June 8, 1976

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Qualifier:

according to guideline

Guideline:

other: Ciba Geigy internal method 15

Deviations:

not specified

Principles of method if other than guideline:

The biological oxygen demand BOD is the amount of oxygen needed to degrade organic substances present in waste water with the help of bacteria (and other microorganisms present in sludge). The expression BOD5 represents the oxygen consumption within 5 days of incubation at 20 °C. Various dilutions of a substance solution in oxygen saturated water will be measured for its oxygen content before and after such an incubation period.

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

ca. 5 d

Initial conc.:

ca. 10 mg/L

Based on:

other: TOC

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- pH:7.2

Reference substance:

not specified

Parameter:

other: BOD5

Value:

ca. 320

Sampling time:

5 d

Remarks on result:

other: results exspressed in mg O2/g

Parameter:

BOD5

Value:

ca. 320 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

ca. 27.9 other: dimensionless value

From each sample three BOD-vessels are prepared in parallel. Two vessels are incubated for 5 days in the incubator, the third one is used to measure the start-value. This vessel is immediately added with 0.5 ml MnCl2 (60 % solution) and 0.5 ml NaOH (30 % solution) and closed with a stopper without inclusion of bubbles.

Subsequently the solution is mixed gently. After sedimentation of flocculants (about 1 hour) the clear supernatant is removed and the sediment is added with:

- 0.2 ml of a 3 % NaN3 solution

- 0.5 ml of a 50 % KJ solution

- 2.0 ml phosphoric acid (density orthophosphoric acid 85 % at 20 °C: δ = 1.71)

After 5 minutes the sediment has resolved again and the produced iodine is titrated with 0.01 N Na2S2O3. At the end of the titration curve starch-solution is added as an indicator solution and the titration is continued until the solution has cleared its colour. All other samples are handled equivalently.

Validity criteria fulfilled:

not specified

Interpretation of results:

not readily biodegradable

Conclusions:

BOD5= 320 mg/g O2

Executive summary:

The substance has been tested for screening test of BOD5 according to the internal method Ciba Geigy n° 15. The substance shows a BOD5=320 mg/g O2

Reference 2

Endpoint:

biodegradation in water: screening test, other

Remarks:

COD

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

June 8, 1976

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

abstract

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: Ciba Geigy internal method 16

Deviations:

not specified

Principles of method if other than guideline:

Within this procedure chemical oxygen demand (COD) is defined as the equivalent amount of oxygen in terms of amount of potassium dichromate that is consumed by reacting with the oxidizable components of 1 g of sample material dissolved in 1 liter water under the given reaction conditions.

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

ca. 5 d

Initial conc.:

ca. 10 mg/L

Based on:

other: TOC

Parameter followed for biodegradation estimation:

O2 consumption

Parameter:

other: COD

Value:

ca. 1 146.88

Sampling time:

5 d

Remarks on result:

other: results exspressed as mg O2/g

Parameter:

COD

Value:

ca. 1 146.88 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

ca. 27.89 mg O2/g test mat.

Calculation of the chemical oxygen demand:

[(a-b) x N x 8.0 x 1000]/v= mg/g O2

a = oxygen consumption [mL] 0.12 n (NH4)2Fe(SO4)2 x 6 H2O for blank sample(s)

b = oxygen consumption [mL] 0.12 n (NH4)2Fe(SO4)2 x 6 H2O for actual sample(s)

N = normality of the (NH4)2Fe(SO4)2 x 6 H2O solution

V = sample volume in [mL] calculated based on the number of dilution steps

Validity criteria fulfilled:

not specified

Interpretation of results:

not readily biodegradable

Conclusions:

COD = 1146.88 mg/g O2

Executive summary:

The substance has been tested for screening test of COD according to the internal method Ciba Geigy n° 16. The substance shows a COD=1146.88 mg/g O2

Description of key information

Not readily biodegradable.