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Long-term toxicity to aquatic invertebrates

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Description of key information

The 14 and 21-Day EL50* (immobilisation) values, based on nominal loading rates, for the parental Daphnia generation (P1) were calculated to be 53 and 37 mg/L Loading rate WAF with 95% confidence limits of 31 - 120 mg/L and 21 - 75 mg/L Loading rate WAF respectively. The 21-Day EL50* (reproduction) value based on nominal loading rates was calculated to be 20 mg/L Loading rate WAF with 95% confidence limits of 8.7 - 59 mg/L Loading rate WAF. The "Lowest Observed Effect Concentration" was considered to be 10 mg/L Loading rate WAF on the basis that at this test concentration significantly fewer live young per adult ( P<0.05) were produced when compared to the control. The "No Observed Effect Concentration" was considered to be 3.2 mg/L Loading rate WAF on the basis that at this test concentration there were no significant mortalities (immobilisation) observed in the parental generation (P1) and that there were no significant differences (P≥0.05) between the control and the 3.2 mg/L Loading rate WAF test group in terms of numbers of live young produced per adult by Day 21.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
3.2 mg/L

Additional information

A key study was performed to assess the effect of the test material on the reproduction of Daphnia magna over a 21-day period. The method followed that described in the OECD Guidelines for Testing of Chemicals No 211 (1998) "Daphnia magna, Reproduction Test", referenced as Method C.20 of Commission Directive 2001/59/EC (which constitutes Annex V of Council Directive 67/548/EEC).

Based on the results of a preliminary range-finding test, Daphnia magna were exposed (10 replicates of a single daphnid per group) to a Water Accommodated Fraction (WAF) of the test material over a range of loading rates of 1.0, 3.2, 10, 32 and 100 mg/L Loading rate WAF for a period of 21 days. The WAFs were renewed 3 times per week. The numbers of live and dead adult Daphnia and young daphnids (live and dead) were determined daily. The Daphnia were fed daily with an algal suspension.

The 14 and 21-Day EL50* (immobilisation) values, based on nominal loading rates, for the parental Daphnia generation (P1) were calculated to be 53 and 37 mg/L Loading rate WAF with 95% confidence limits of 31 - 120 mg/L and 21 - 75 mg/L Loading rate WAF respectively. The 21-Day EL50* (reproduction) value based on nominal loading rates was calculated to be 20 mg/L Loading rate WAF with 95% confidence limits of 8.7 - 59 mg/L Loading rate WAF. The "Lowest Observed Effect Concentration" was considered to be 10 mg/L Loading rate WAF on the basis that at this test concentration significantly fewer live young per adult ( P<0.05) were produced when compared to the control. The "No Observed Effect Concentration" was considered to be 3.2 mg/L Loading rate WAF on the basis that at this test concentration there were no significant mortalities (immobilisation) observed in the parental generation (P1) and that there were no significant differences (P≥0.05) between the control and the 3.2 mg/L Loading rate WAF test group in terms of numbers of live young produced per adult by Day 21.

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