Isobutyl 4-hydroxybenzoate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro cytogenicity / chromosome aberration study in mammalian cells

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data from peer review publication

Data source

Reference

Reference Type:

publication

Title:

CHROMOSOME TESTS WITH 134 COMPOUNDS ON CHINESE HAMSTER CELLS IN VITRO -- A SCREENING FOR CHEMICAL CARCINOGENS

Author:

MOTOI ISHIDATE, Jr. and SHIGEYOSHI ODASHIMA

Year:

1977

Bibliographic source:

Mutation Research, 48 (1977) 337—354

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mer mentiond below

Principles of method if other than guideline:

In vitro chromosomal aberration was conducted on Chinese hamster fibroblast cell line to evaluate genetoxicity effects of chemical.

GLP compliance:

no

Type of assay:

gene mutation assay in fungi

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyl 4-hydroxybenzoate

EC Number:

224-208-8

EC Name:

Isobutyl 4-hydroxybenzoate

Cas Number:

4247-02-3

Molecular formula:

C11H14O3

IUPAC Name:

2-methylpropyl 4-hydroxybenzoate

Details on test material:

- Name of test material: Isobutyl 4-hydroxybenzoate
- Molecular formula: C11H14O3
- Molecular weight: 194.23 g/mol
- Substance type: Organic
- Physical state: Solid

Method

Target gene:

Chinese hamster fibroblast cell line

Species / strain

Species / strain / cell type:

other: Chinese hamster fibroblast cell line

Details on mammalian cell type (if applicable):

- Type and identity of media: The cells had been maintained by 5-day passages and grown in a monolayer in petri dishes with Eagle's MEM (GIBCO F-11) supplemented with 10% calf serum

Additional strain / cell type characteristics:

not specified

Metabolic activation:

not specified

Metabolic activation system:

no data

Test concentrations with justification for top dose:

0.03%

Vehicle / solvent:

- Vehicle(s)/solvent(s) used:Ethanol

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

not specified

Positive control substance:

not specified

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period:48-h

Evaluation criteria:

% aberration

Results and discussion

Test results

Species / strain:

other: Chinese hamster fibroblast cell line

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

No data

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Negative effects were known in chromosomal aberration test when tested in Chinese hamster fibroblast cell line with dose concentration of 03.% in ethanol.

Executive summary:

Chromosomal aberration test was conducted onChinese hamster fibroblast cell line for the chemical Isobuyl paraben.The Cells were treated with colcemid (0.2 pg/ml) for 2 h, and after trypsinization, they were incubated in 0.075 M KC1 hypotonic solution for 15 min. at 37°C.The dose concentration was 0.3% .

The test produced no chromosomal aberration after 48-h.Hence,Negative effects were known in chromosomal aberration test when tested inChinese hamster fibroblast cell line with dose concentration of 03.% in ethanol.