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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

Direct Blue 273 is a dye and this may impact the experiments conducted on algae. For this reason, it has been judged as more appropriate in the assessment to use a study on Lemna aquatic plant.

Key value for chemical safety assessment

Additional information

No data on Direct Blue 273 was available, thus the assessment was based on a read across approach, using Similar Substance 01, i.e. an isomer of Direct Blue 273 differing only for the position of a functional group on an aromatic ring.

In the available study on algae based on EU method C.3, effects of direct and indirect exposure to Similar Substance 01 were within 10 % at all concentrations, thus demonstrating that the colour of test solution may contribute to the final outcome. Consequently, this result was not used in the assessment.