Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 277-955-7 | CAS number: 74664-50-9
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- other: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The complete read across justification is detailed in section 13. Test substance is an isomer of the substance under registration; structural difference is not expected to significantly impact the toxicity to microorganisms. Source study has reliability 2: only limited information available from a migrated NONS file, as per Article 25(3) request.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Water media type:
- not specified
- Total exposure duration:
- 3 h
- Dissolved oxygen:
- Aerobic conditions.
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- not specified
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- < 1 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- not specified
- Validity criteria fulfilled:
- not specified
- Conclusions:
- IC50(3h) > 10000 mg/l
- Executive summary:
Method
The test has been conducted according to the OECD guideline 209, in compliance to the GLP principles. Available information derived from migrated NONS file, as per Article 25(3) request, with permission to refer granted by ECHA.
Results
IC50(3h) > 10000 mg/l
Reference
Description of key information
IC50 (3h) > 10000 mg/l
Key value for chemical safety assessment
Additional information
No data on Direct Blue 273 was available, thus the assessment was based on a read across approach, using Similar Substance 01, i.e. an isomer of Direct Blue 273 differing only for the position of a functional group on an aromatic ring.
A test was carried out according to OECD guideline 209. Based on the structural similarity, the result obtained with Similar Substance 01 was considered as representative for the potential toxicity of Direct Blue 273 to microorganisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
