Sodium 4-vinylbenzenesulphonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14-28 October 1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Principles of method if other than guideline:

Test sample, suspended in an appropriate solvent was dosed to test animals by oral gavage. The LD50 was determined according to the Litchfield-Wilcoxon method method. Test procedure is consistent with the requirements of the OECD 401 method.

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 4-vinylbenzenesulphonate

EC Number:

220-266-3

EC Name:

Sodium 4-vinylbenzenesulphonate

Cas Number:

2695-37-6

Molecular formula:

C8H8O3S.Na

IUPAC Name:

.

Test animals

Species:

mouse

Strain:

ICL-ICR

Sex:

male

Details on test animals or test system and environmental conditions:

The mice were kept in the animal room, where temperature was controlled at 22±0.5 oC and its relative humidity was controlled 60±5%. The mice took solid feed CE-2 (CLEA Japan) and drank tap water ab libium

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

Since sodium p-styrenesulfonate was solid, the test substance was suspended into olive oil to prepare 40 %(w/v) slurry and used for the test.

Doses:

The applied doses were set at 16.00, 13.92, 12.10, 10.52 and 9.14 g/kg

No. of animals per sex per dose:

10 male animals per dose

Control animals:

not specified

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

>= 6 400 mg/kg bw

Based on:

test mat.

Remarks:

A purity correction has been applied to correct for the vehicle content.

Mortality:

Tests were conducted at technically maximum dose levels, 16.00, 13.92, 12.10, 10.52 and 9.14 g/kg at 40% in olive oil, but no mortality of the test animal was observed at any dose levels.

Clinical signs:

Decrease of spontaneous motion and piloerection were observed after treatment, but the symptoms were disappeared in the next day. Dose groups at 16.00, 13.92 and 12.10 g/kg, light diarrhea were observed.

Gross pathology:

At necropsy at the end of test, sodium p-styrenesulfonate treatment group gave no obvious change observations.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Assigning a correction for the content of vehicle, the LD50 of the substance is >6400 mg/kg

Executive summary:

The acute oral toxicity of the substance has been assessed following dosing of male ICR-SLC mice at dose levels upto 16 g/kg with a dose concentration of 40% in olive oil. Correcting for vehicle content, the maximum dose was 6400 mg/kg of test substance. Dosing was conducted by oral gavage using a test method consistent with the classification OECD 401 style of acute oral toxicity testing, but not in compliance with GLP. No mortality was observed upto the maximum dose. The LD50 >6400mg/kg.