1-(1,1-dimethylethoxy)-2-methylpropane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-(1,1-dimethylethoxy)-2-methylpropane

EC Number:

251-347-1

EC Name:

1-(1,1-dimethylethoxy)-2-methylpropane

Cas Number:

33021-02-2

Molecular formula:

C8H18O

IUPAC Name:

1-(tert-butoxy)-2-methylpropane

Details on test material:

- Name of test substance : Isobutyl-tert. Butylether (IBTBE)
- Test substance No .: 01/0084-1
- Chemical name: Propane, 1-(1,1 dimethyl-ethoxy)-2-methyl-
- CAS-No. 33021-02-2
- Batch No.: Sumpf K 121
- Date of production: Feb 06, 2001
- Physical state / appearance : Liquid, colourless - clear
- Purity: 86.4 % (information by the sponsor)
- Homogeneity: Homogeneous

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The test concentrations were freshly prepared every 48 hours. The preparation was carried out separately for each test vessel . All flasks and vessels needed were rinsed with test solution before use. The test substance was weighed into a flask, an alliquot of the test water was added and mixed with the test substance by shaking thoroughly. The mixture was poured into the test vessel, the flask was refilled with test water and poured into the test vessel to ensure complete transfer of test substance into the test vessel. This procedure was repeated until a final volume of 10 L was achieved.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Test species / strain: Zebrafish (Danio rerio)
- Animal supplier: Zierfisch-Center Klöckner, Ludwigshafen, Germany
- Fish weight: 0.18 g(0.15 - 0.32 g)
- Fish length: 2.8 cm (2 .6 - 3.4 cm)
- Age: Approx. 2 months
- Physical conditions: No abnormalities

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Approx. 2.5 mmol/L = 250 mg/L CaCO3

Test temperature:

23°C

pH:

7.9 - 8.4

Dissolved oxygen:

5.1 - 8.7 mg/L

Details on test conditions:

- Test vessels : 10 L Glass flasks, closed during exposure intervals
- Volume of water: 10L
- Animals / test vessel: 10
- Loading (g fish/L water): 0.18
- Test vessel / concentration : 1
- Photoperiod : 16 hours light, 8 hours darkness
- Temperature: 23°C
- Aeration: None
- Feeding: None

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

64.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes