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EC number: 251-347-1 | CAS number: 33021-02-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Isobutyl-tert.Butylether (IBTBE)
- IUPAC Name:
- Isobutyl-tert.Butylether (IBTBE)
- Details on test material:
- - Test substance: Isobutyl-tert.Butylether (IBTBE)
- purity: 86.4%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen .
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Details on test conditions:
- - Test vessel : glass tubes sealed with gas unpermeable teflone caps (nominal volume 20 ml )
- Test volume : approx. 23 ml (brimful)
- Loading (animals/ml) : approx. 0,22
- Number of animals/vessel : 5
- Total number of animals/conc. : 20
- Number of replicates/conc . : 4
- Number of replicates/control : 4
- Number of replicates for the measurement of pH and 02 : 1
- Number of parallels without animals: 1(parallel 5) per test concentration and control for measurement of the pH and oxygen content at the beginning of the exposition. This parallel was thrown away after measurement. 4 per test concentration and control for concentration control analysis .
- Age of the animals at the begin of exposition : 2 - 24 h (starting with the 3rd breed of parent animals)
- Age of stock animals : 2 - 4 weeks
- Illumination : Artificial light, type warm white (e .g. OSRAM L58 W31)
- day:night rhythm = 16 : 8 h
- Light intensity: .About 1- 8 NE/(mZ•s) at a wave length of 400 - 750 nm
- Test parameter : Swimming ability of the test animals
- Check of the study and recording (swimming ability) : Visually after 0, 24 and 48 h
- Feeding : None
- Measurement of temperature : Continuously during the whole test period in a separate vessel close the test vessels .
- Measurement of pH and oxygen : After Oh in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration . - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.11 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 1.75 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Any other information on results incl. tables
EC0 (48h) = 25 mg/l nominal (*0.07=1.75 mg/l effective)
EC50 (48h) = 58.7 mg/l nominal (*0.07=4.11 mg/l effective)
EC100 (48h) = 100 mg/l nominal (*0.07=7.0 mg/l effective)
24 h(nominal): EC0=25mg/l, EC50=60.4mg/l, EC100>100mg/l;
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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