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Ecotoxicological information

Toxicity to terrestrial arthropods

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Reference
Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
C12 LAS; Linear Alkylbenzene Sulfonate (LAS)) is a very close analogue of Calcium dodecylbenzenesulfonate (CAS No 26264-06-2, EC Number; 247-557-8) ) and read-across is valid.
Reason / purpose for cross-reference:
read-across source
Qualifier:
no guideline followed
Principles of method if other than guideline:
The toxicity of LAS to soil dwelling organisms was tested in three soil types. Various LAS salts (Na, Ca, and Mg) were tested in one soil type (sandy) to determine the effection of speciation to toxicity. In each test, 10 male and 10 female organisms were placed in containers with test substance added to the soil. Exposure lasted 21 days, at the end of which the number of adult and juvenile organisms were counted.
GLP compliance:
no
Application method:
soil
Analytical monitoring:
yes
Details on sampling:
30 g soil samples were stored at -20 degrees C until analysis
Vehicle:
yes
Details on preparation and application of test substrate:
Acetone was used in the Ca-LAS and Mg-LAS tests as these compounds are insoluble in water.
Test organisms (species):
Folsomia sp.
Animal group:
Collembola (soil-dwelling springtail)
Details on test organisms:
TEST ORGANISM
- Source: laboratory culture
- Age at test initiation (mean and range, SD): 23-26 days
- Weight at test initiation (mean and range, SD):
Study type:
laboratory study
Total exposure duration:
21 d
Test temperature:
20 degrees C
pH (if soil or dung study):
5.5-5.8
Photoperiod and lighting:
12:12 photoperiod
Details on test conditions:
TEST SYSTEM
- Test container (material, size): 5.5 x 6 cm cylindrical containers
- Amount of soil or substrate: 30 g moist soil
- No. of organisms per container (treatment): 10 male/10 female

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Lundgaard
- Soil texture (if natural soil)
- % sand: 66.9 coarse, 15.8 fine
- % silt: 8.6
- % clay: 6.2

- Soil classification system: sandy soil
- Organic carbon (%): 1.5
- CEC: 3.23 mEq/100 g
- Pretreatment of soil: dried at 80 degrees C, and sifted through 2 mm mesh


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Askov
- Soil texture (if natural soil)
- % sand: 38.4 coarse, 23.6 fine
- % silt: 22.3
- % clay: 13.0

- Soil classification system: loamy soil
- Organic carbon (%): 1.6
- CEC: 8.14 mEq/100 g
- Pretreatment of soil: dried at 80 degrees C, and sifted through 2 mm mesh
OTHER TEST CONDITIONS
- Photoperiod: 12:12


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Flakkebjerg
- Soil texture (if natural soil)
- % sand: 23.4 coarse, 25.5 fine
- % silt: 34.2
- % clay: 15.1

- Soil classification system: clay soil
- Organic carbon (%): 1.0
- CEC: 10.16 mEq/100 g
- Pretreatment of soil: dried at 80 degrees C, and sifted through 2 mm mesh

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : number of surviving adults and juveniles counted on day 21
Nominal and measured concentrations:
0, 8, 28, 79, 278, 793 mg/kg dry weigth measured
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
85 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Na-LAS in sandy soil
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
93 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Na-LAS in clay soil
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
87 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Na-LAS in sandy soil
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
106 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Ca-LAS in sandy soil
Duration:
21 d
Dose descriptor:
EC10
Effect conc.:
97 mg/kg soil dw
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: Mg-LAS in sandy soil
Details on results:
Soil type had little effect on the toxicity of Na-LAS to soil organisms. Salt speciation also had little effect on toxicity.
Validity criteria fulfilled:
yes
Conclusions:
The EC10 for Folsomia fimetaria in various soils ranged from 85-93 mg/kg dry weight soil.
Executive summary:

Groups of 10 male and 10 female Folsomia fimetaria were exposed to various concentrations of Na-LAS in three different soil types for 21 days. At the end of this period, the number of adult and juvenile organisms was counted. Another test was done to compare the toxicity of three LAS salts, Na, Ca, and Mg, in one soil type (sandy). There was little variation in toxicity among the three soil types and in the three salt species. The EC10 for Folsomia fimetaria in various soils ranged from 85-93 mg/kg dry weight soil

Description of key information

Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted. There was no effect on adult survival, however, there was a dose-related decrease in reproduction. 
The EC10 based on reproduction was 147 mg/kg dw soil.
Groups of 10 male and 10 female Folsomia fimetaria were exposed to various concentrations of Na-LAS in three different soil types for 21 days. At the end of this period, the number of adult and juvenile organisms was counted. Another test was done to compare the toxicity of three LAS salts, Na, Ca, and Mg, in one soil type (sandy). There was little variation in toxicity among the three soil types and in the three salt species.
The EC10 for Folsomia fimetaria in various soils ranged from 85-93 mg/kg dry weight soil

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil dwelling arthropods:
425 mg/kg soil dw
Long-term EC10, LC10 or NOEC for soil dwelling arthropods:
85 mg/kg soil dw

Additional information

Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted. There was no effect on adult survival, however, there was a dose-related decrease in reproduction.

The EC10 based on reproduction was 147 mg/kg dw soil.

 

Groups of 10 male and 10 female Folsomia fimetaria were exposed to various concentrations of Na-LAS in three different soil types for 21 days. At the end of this period, the number of adult and juvenile organisms was counted. Another test was done to compare the toxicity of three LAS salts, Na, Ca, and Mg, in one soil type (sandy). There was little variation in toxicity among the three soil types and in the three salt species.

The EC10 for Folsomia fimetaria in various soils ranged from 85-93 mg/kg dry weight soil

 

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