Reaction Mass of disodium [(E)-7-nitro-3-oxido-4-((2-oxidonaphthalen-1-yl)diazenyl)naphthalene-1-sulfonate) (E)-1-((3,5-dinitro-2-oxidophenyl)diazenyl)naphthalen-2-olate) chromate(2-)] and Hydrogen [(E)-7-nitro-3-oxido-4-((2-oxidonaphthalen-1-yl)diazenyl)naphthalene-1-sulfonate)(E)-1-((3,5-dinitro-2-oxidophenyl)diazenyl)naphthalen-2-olate) chromate(2-)]

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Supplier: Charles River Aquatics, Someren, NetherlandsThe quality of the fish was checked with an acclimatization time of 61 days in dechlorinated tap water.For each concentration, 7 fish were used. The fish were not fed during the test.

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

142 mg/l CaCO3

pH:

7.9-8.4

Dissolved oxygen:

95-96%

Nominal and measured concentrations:

0.08, 0.18, 0.40, 0.88, 1.9 and 4.3 mg/l. Additionally a blank control without test substance was tested.

Details on test conditions:

Light: daylightApparatus: static test in 5 litre glass aquaria with a test volume of 3 litre, slowly aerated

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 0.66 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

In the test concentrations up to 0.40 mg/1 no alteration of the test substance in the test media was observed during the test. In the concentrations of 0.88 up to 4.3 mg/1 a small sediment at the bottom of the test vessels was observed after 24 hours.

Sublethal observations / clinical signs:

nominal concentration (mg/l)	24h	48h	72h	96h
blank	0	0	0	0
0.08	0	0	0	0
0.18	0	0	0	0
0.40	1	2	2	2
0.88	2	4	4	4
1.9	7	7	7	7
4.3	7	-	-	-

Conclusions:

The test item was assessed for acute toxicity to fish following OECD 203. Under the experimental conditions the LC50, 96h ca. 0.66 mg/l.

Executive summary:

The test item was assessed for acute toxicity to fish following OECD 203. Seven zebra fish were tested for each nominal concentration, including a blank reference, namely 0.08, 0.18, 0.40, 0.88, 1.9 and 4.3 mg/l with an exposure time of 96h. Starting from 0.88 mg/l slight precipitation was observed at the bottom of the vessel. Mortality and behaviour were assessed and the LC50, 96h resulted equal to 0.66 mg/l.

Description of key information

zebra fish, 96h, LC50 = 0.66 mg/l

Additional information

Acute toxicity to fish was tested following OECD 203 (Huntsman, 1998) on the analogue substance.. Very low nominal concentrations were assessed, however some precipitations occured at the two highest doses and mortality was neverthless observed, showing a LC50,96h equal to 0.66 mg/l.

Based on the read across considerations the same results apply to Acid Black 107:1