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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Annex V
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
444-140-4
EC Name:
-
Cas Number:
18934-00-4
Molecular formula:
C12H22O3
IUPAC Name:
3-ethyl-3-{[(3-ethyloxetan-3-yl)methoxy]methyl}oxetane
Test material form:
liquid
Specific details on test material used for the study:
Nature of substance: undiluted liquid

Test animals / tissue source

Species:
other: Rabbit (New Zealand White)

Test system

Amount / concentration applied:
1 mL
Number of animals or in vitro replicates:
3

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
24/48/72 h
Score:
ca. 2
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
24/48/72 h
Score:
ca. 1.67
Irritation parameter:
conjunctivae score
Basis:
animal #3
Time point:
24/48/72 h
Score:
ca. 1.33
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
24/48/72 h
Score:
ca. 1.33
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
24/48/72 h
Score:
ca. 1
Irritation parameter:
conjunctivae score
Basis:
animal #3
Time point:
24/48/72 h
Score:
ca. 0.33
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
24/48/72 h
Score:
ca. 1
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
24/48/72 h
Score:
ca. 0.67
Irritation parameter:
conjunctivae score
Basis:
animal #3
Time point:
24/48/72 h
Score:
ca. 0
Irritation parameter:
iris score
Basis:
animal #1
Time point:
24/48/72 h
Score:
ca. 0.67
Irritation parameter:
iris score
Basis:
animal #2
Time point:
24/48/72 h
Score:
ca. 0.33
Irritation parameter:
iris score
Basis:
animal #3
Time point:
24/48/72 h
Score:
ca. 0
Irritant / corrosive response data:
Reversibility of any observed effect: Changes fully reversible within 7 days
Other effects:
Scattered or diffuse corneal opacity was noted in two
treated eyes at the 24 and 48-hour observations and
persisted in one treated eye at the 72-hour observation.
Iridial inflammation was noted in all treated eyes one hour
after treatment, in two treated eyes at the 24-hour
observation and persisted in one treated eye at the 48-hour
observation.
Moderate conjunctival irritation was noted in all treated
eyes one and 24 hours after treatment.
Moderate conjunctival irritation was noted in two treated
eyes with minimal conjunctival irritation in one treated eye
at the 48-hour observation. Moderate conjunctival
irritation was noted in one treated eye with minimal
conjunctival redness in two treated eyes at the 72-hour
observation.
All treated eyes appeared normal at the 7-day observation.

Applicant's summary and conclusion

Interpretation of results:
other: not classified