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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Annex V (Ames).
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
444-140-4
EC Name:
-
Cas Number:
18934-00-4
Molecular formula:
C12H22O3
IUPAC Name:
3-ethyl-3-{[(3-ethyloxetan-3-yl)methoxy]methyl}oxetane
Test material form:
liquid

Method

Species / strain
Species / strain / cell type:
bacteria, other: Salmonella typhimurium: TA1535, TA1537, TA98 and TA100. Escherichia coli WP2 uvrA
Metabolic activation system:
Phenobarbital and 5,6-benzoflavone induced, rat-liver S9.
Test concentrations with justification for top dose:
Concentration range in the main test (with metabolic activation): 156 ... 5000 μg/plate
Concentration range in the main test (without metabolic activation): 156 ... 5000 μg/plate
Vehicle / solvent:
Solvent: Dimethyl sulfoxide

Results and discussion

Test resultsopen allclose all
Species / strain:
other: as specified above
Metabolic activation:
with and without
Genotoxicity:
not specified
Remarks:
NA
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
5000 μg/plate
Species / strain:
other: as specified above
Metabolic activation:
with and without
Genotoxicity:
not specified
Remarks:
NA
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
2500 μg/plate

Applicant's summary and conclusion

Conclusions:
negative with metabolic activation
negative without metabolic activation