Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Referenceopen allclose all

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 8.0 G, 9.2 CM, 0.8 G, 4.6 CM,7.9 G, 9.3 CM
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 100000 NR TO NR 100000 ug/L NR
F 70000 NR TO NR 70000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 100000 NR TO NR 100000 ug/L NR
F 70000 NR TO NR 70000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isobutyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 20 mg/l (NOEC). One of the 96h-LC50 was 100 mg/l
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
70 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isobutyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 14mg/l (NOEC). One of the 96h-LC50 was 70 mg/l
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isobutyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 14mg/l (NOEC). One of the 96h-LC50 was 70 mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)

Preliminary testing of eight collectors (xanthates) and four frothers in 96-h static and 28-day flow-through bioassays using rainbow trout as the test organism show a great disparity in the toxicity of the chemicals administered in these two ways.

For the short-term tests, the relative toxicity of the compounds is expressed as an lc50 or as a range of concentration in mg l-1 in which the lc50 is expected to fall. Of the collectors tested in this way sodium ethyl and potassium amyl xanthate were the most toxic, with lc50's in the range of 30-50 mg l-1. Among the frothers, xylenol (cresylic acid) was found to be the most toxic (5.6 mg l-1 >lc50 > 3.2 mg l-1) while polypropylene glycol was least toxic (lc50 > 1000 mg l-1).

The long-term tests using potassium ethyl, sodium isopropyl, sodium ethyl, and potassium amyl xanthate indicated that in the flow-through system, the toxicity of the chemicals was in the order of 100 fold greater compared with the static bioassay results.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium O-isobutyl dithiocarbonate (SIBX) as a main constituent of Reaction mass of 2-methylpropan-1-ol and sodium O-isobutyl dithiocarbonate and sodium hydroxide induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 20 mg/l (NOEC). One of the 96h-LC50 was 100 mg/l
Under the conditions of the present test Sodium isobutyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 14mg/l (NOEC). One of the 96h-LC50 was 70 mg/l
Executive summary:

Under the conditions of the present test Sodium O-isobutyl dithiocarbonate (SIBX) as a main constituent of Reaction mass of 2-methylpropan-1-ol and sodium O-isobutyl dithiocarbonate and sodium hydroxide induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 20 mg/l (NOEC). One of the 96h-LC50 was 100 mg/l

Under the conditions of the present test Sodium isobutyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 14mg/l (NOEC). One of the 96h-LC50 was 70 mg/l

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: 9.0 G
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 70000 NR TO NR 70000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 70000 NR TO NR 70000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
70 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isobutyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 14mg/l (NOEC). One of the 96h-LC50 was 70 mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
GENERAL COMMENTS: LOC/NR// FOLLOWED APHA STANDARD METHODS// DO/1 MG/L < SATURATION// AERATION/
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium O-isobutyl dithiocarbonate (SIBX) as a main constituent of Reaction mass of 2-methylpropan-1-ol and sodium O-isobutyl dithiocarbonate and sodium hydroxide induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 14mg/l (NOEC). One of the 96h-LC50 was 70 mg/l
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to sodium O-sec-butyl dithiocarbonate
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 2.6 G, 6.0 CM, 0.9 G, 4.6 CM,3.5 G, 6.5 CM
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 100000 NR TO NR 100000 ug/L NR
F 166000 NR TO NR 166000 ug/L NR
F 320000 NR TO NR 320000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 100000 NR TO NR 100000 ug/L NR
F 166000 NR TO NR 166000 ug/L NR
F 320000 NR TO NR 320000 ug/L NR
WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium sec-butyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 20 mg/l (NOEC). One of the 96h-LC50 was 100 mg/l
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
166 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium sec-butyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 33.2mg/l (NOEC). One of the 96h-LC50 was 166 mg/l
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium sec-butyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 64mg/l (NOEC). One of the 96h-LC50 was 320 mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)

Preliminary testing of eight collectors (xanthates) and four frothers in 96-h static and 28-day flow-through bioassays using rainbow trout as the test organism show a great disparity in the toxicity of the chemicals administered in these two ways.

For the short-term tests, the relative toxicity of the compounds is expressed as an lc50 or as a range of concentration in mg l-1 in which the lc50 is expected to fall. Of the collectors tested in this way sodium ethyl and potassium amyl xanthate were the most toxic, with lc50's in the range of 30-50 mg l-1. Among the frothers, xylenol (cresylic acid) was found to be the most toxic (5.6 mg l-1 >lc50 > 3.2 mg l-1) while polypropylene glycol was least toxic (lc50 > 1000 mg l-1).

The long-term tests using potassium ethyl, sodium isopropyl, sodium ethyl, and potassium amyl xanthate indicated that in the flow-through system, the toxicity of the chemicals was in the order of 100 fold greater compared with the static bioassay results.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium sec-butyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 20 mg/l (NOEC). One of the 96h-LC50 was 100 mg/l
Under the conditions of the present test Sodium sec-butyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 33.2mg/l (NOEC). One of the 96h-LC50 was 166 mg/l
Under the conditions of the present test Sodium sec-butyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 64mg/l (NOEC). One of the 96h-LC50 was 320 mg/l
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to sodium O-sec-butyl dithiocarbonate
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: 0.9 G
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
>1 mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 166000 NR TO NR 166000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 166000 NR TO NR 166000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: >1 NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
166 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isobutyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 33.2 mg/l (NOEC). One of the 96h-LC50 was 166 mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium isobutyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 33.2mg/l (NOEC). One of the 96h-LC50 was 166 mg/l
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to potassium O-hexyl dithiocarbonate
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 3.5 G, 6.5 CM
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F NR 10000 TO 100000 10000 TO 100000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F NR 10000 TO 100000 10000 TO 100000ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
100 - 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Potassium hexyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 20 mg/l (NOEC). One of the 96h-LC50 was between 100 and 1000 mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)

Preliminary testing of eight collectors (xanthates) and four frothers in 96-h static and 28-day flow-through bioassays using rainbow trout as the test organism show a great disparity in the toxicity of the chemicals administered in these two ways.

For the short-term tests, the relative toxicity of the compounds is expressed as an lc50 or as a range of concentration in mg l-1 in which the lc50 is expected to fall. Of the collectors tested in this way sodium ethyl and potassium amyl xanthate were the most toxic, with lc50's in the range of 30-50 mg l-1. Among the frothers, xylenol (cresylic acid) was found to be the most toxic (5.6 mg l-1 >lc50 > 3.2 mg l-1) while polypropylene glycol was least toxic (lc50 > 1000 mg l-1).

The long-term tests using potassium ethyl, sodium isopropyl, sodium ethyl, and potassium amyl xanthate indicated that in the flow-through system, the toxicity of the chemicals was in the order of 100 fold greater compared with the static bioassay results.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Potassium hexyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 20 mg/l (NOEC). One of the 96h-LC50 was between 100 and 1000 mg/l
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to SIPX
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 1-10 G
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
348 mg/L CaCO3
ALKALINITY: 203 mg/L CaCO3
Test temperature:
12 0C
pH:
8.6
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F NR 18000 TO 20 000 18000 TO 20000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F NR 18000 TO 20 000 18000 TO 20000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 12 NR TO NR C
pH: 8.6 NR TO NR
HARDNESS: 348 NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: 203 NR TO NR mg/L CaCO3
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
18 - 20 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 3.6mg/l (NOEC). One of the 96h-LC50 was between 18 and 20 mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
GENERAL COMMENTS: LOC/NR// HARD,ALK,PH/OF DILUTION H2O// AERATED//
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 3.6mg/l (NOEC). One of the 96h-LC50 was between 18 and 20 mg/l
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to SIPX
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 2.0 G, 5.8 CM,< 10 G
Test type:
other: static and flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 217000 NR TO NR 217000 ug/L NR
F 300 NR TO NR 300 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static), F (flow-through)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s),72 hour(s)
STAND DUR. (D): 4 day(s,3 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 217000 NR TO NR 217000 ug/L NR
F 300 NR TO NR 300 ug/L NR


WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
217 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
static conditions
Remarks on result:
other: Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 43.4mg/l (NOEC). One of the 96h-LC50 was 217 mg/l
Duration:
72 h
Dose descriptor:
LC100
Effect conc.:
0.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
flow-through conditions
Remarks on result:
other: Under the conditions of the present test Sodium isopropyl xanthate induced 100% mortality in Oncorhynchus mykiss (Rainbow Trout) in flow-through conditions at 0.3 mg/l. One of the72h-LC100 was 0.3 mg/l.
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
180 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
static conditions
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)

Preliminary testing of eight collectors (xanthates) and four frothers in 96-h static and 28-day flow-through bioassays using rainbow trout as the test organism show a great disparity in the toxicity of the chemicals administered in these two ways.

For the short-term tests, the relative toxicity of the compounds is expressed as an lc50 or as a range of concentration in mg l-1 in which the lc50 is expected to fall. Of the collectors tested in this way sodium ethyl and potassium amyl xanthate were the most toxic, with lc50's in the range of 30-50 mg l-1. Among the frothers, xylenol (cresylic acid) was found to be the most toxic (5.6 mg l-1 >lc50 > 3.2 mg l-1) while polypropylene glycol was least toxic (lc50 > 1000 mg l-1).

The long-term tests using potassium ethyl, sodium isopropyl, sodium ethyl, and potassium amyl xanthate indicated that in the flow-through system, the toxicity of the chemicals was in the order of 100 fold greater compared with the static bioassay results.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) in static conditions below 43.4mg/l (NOEC). One of the 96h-LC50 was 217 mg/l.
Under the conditions of the present test Sodium isopropyl xanthate induced 100% mortality in Oncorhynchus mykiss (Rainbow Trout) in flow-through conditions at 0.3 mg/l. One of the72h-LC100 was 0.3 mg/l.
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to SIPX
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Ictalurus punctatus
Details on test organisms:
SPECIES #
(NAME): 20 Ictalurus punctatus (Channel Catfish)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: 1.1 G
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
272 mg/L CaCO3
Test temperature:
15 0C
pH:
7.4
Dissolved oxygen:
NR
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TEST CONCENTRATION
CHEM ANAL. METHOD: not reported
UNIT OF MEASURE: AI mg/L active ingredient milligram per Liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
A 10 NR TO NR 10000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: C (Concurrent control)
EXPOSURE TYPE: S ( static),
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s),72 hour(s)
STAND DUR. (D): 4 day(s,3 day(s

TEST CONCENTRATION
CHEM ANAL. METHOD: not reported
UNIT OF MEASURE: AI mg/L active ingredient milligram per Liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
A 10 NR TO NR 10000 ug/L NR




WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 15 NR TO NR C
pH: 7.4 NR TO NR
HARDNESS: 272 NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: NR NR TO NR NR
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Ictalurus punctatus below 2 mg/l (NOEC). One of the 96h-LC50 was 10mg/l
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Ictalurus punctatus below 2 mg/l (NOEC). One of the 24h-LC50 was 10mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: INC
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT: Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: P (Publication reported endpoint)
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (A): NR ( NR to NR)
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Ictalurus punctatus below 2 mg/l (NOEC). One of the 96h-LC50 was 10mg/l
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to SIPX
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Lepomis macrochirus
Details on test organisms:
SPECIES #
(NAME): 2 Lepomis macrochirus (Bluegill)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: 0.6G
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
272 mg/L CaCO3
Test temperature:
15 0C
pH:
7.4
Dissolved oxygen:
NR
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TEST CONCENTRATION
CHEM ANAL. METHOD: not reported
UNIT OF MEASURE: AI mg/L active ingredient milligram per Liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
A 10 NR TO NR 10000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: C (Concurrent control)
EXPOSURE TYPE: S ( static),
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s),72 hour(s)
STAND DUR. (D): 4 day(s,3 day(s

TEST CONCENTRATION
CHEM ANAL. METHOD: not reported
UNIT OF MEASURE: AI mg/L active ingredient milligram per Liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
A 10 NR TO NR 10000 ug/L NR




WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 15 NR TO NR C
pH: 7.4 NR TO NR
HARDNESS: 272 NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: NR NR TO NR NR
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Lepomis macrochirus below 2 mg/l (NOEC). One of the 96h-LC50 was 10mg/l
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Lepomis macrochirus below 2 mg/l (NOEC). One of the 24h-LC50 was 10mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: INC
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT: Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: P (Publication reported endpoint)
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (A): NR ( NR to NR)
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Sodium isopropyl xanthate induced no mortality in Lepomis macrochirus below 2 mg/l (NOEC). One of the 96h-LC50 was 10mg/l
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to O-isopropyl hydrogen dithiocarbonate
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 3.5 G, 6.5 CM
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F NR 32000 TO 320000 (32000-320000) ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F NR 32000 TO 320000 (32000-320000) ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
32 - 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Potassium isopropyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 6.4 mg/l (NOEC). One of the 96h-LC50 was between 32 and 320 mg/l
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)

Preliminary testing of eight collectors (xanthates) and four frothers in 96-h static and 28-day flow-through bioassays using rainbow trout as the test organism show a great disparity in the toxicity of the chemicals administered in these two ways.

For the short-term tests, the relative toxicity of the compounds is expressed as an lc50 or as a range of concentration in mg l-1 in which the lc50 is expected to fall. Of the collectors tested in this way sodium ethyl and potassium amyl xanthate were the most toxic, with lc50's in the range of 30-50 mg l-1. Among the frothers, xylenol (cresylic acid) was found to be the most toxic (5.6 mg l-1 >lc50 > 3.2 mg l-1) while polypropylene glycol was least toxic (lc50 > 1000 mg l-1).

The long-term tests using potassium ethyl, sodium isopropyl, sodium ethyl, and potassium amyl xanthate indicated that in the flow-through system, the toxicity of the chemicals was in the order of 100 fold greater compared with the static bioassay results.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Potassium isopropyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 6.4 mg/l (NOEC). One of the 96h-LC50 was between 32 and 320 mg/l
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to PAX
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 3.5 G, 6.0-7.0 CM,6.8 G, 8.8 CM
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
118 to 125 mg/L CaCO3
Test temperature:
10 0C
pH:
7.9 to 8.2
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 18000 NR TO NR (18000) ug/L NR
F NR 32000 TO 56000 (32000-56000) ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L ) IONCIC FRACTION
F 18000 NR TO NR (18000) ug/L NR
F NR 32000 TO 56000 (32000-56000) ug/L NR
WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 10 NR TO NR C
pH: NR 7.9 TO 8.2
HARDNESS: NR 118 TO 125 mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
static conditions
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Potassium amyl xanthate induced no mortality (static conditions) in Oncorhynchus mykiss (Rainbow Trout) below 4.5 mg/l (NOEC). One of the 96h-LC50 was 18 mg/l
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
32 - 56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)

Preliminary testing of eight collectors (xanthates) and four frothers in 96-h static and 28-day flow-through bioassays using rainbow trout as the test organism show a great disparity in the toxicity of the chemicals administered in these two ways.

For the short-term tests, the relative toxicity of the compounds is expressed as an lc50 or as a range of concentration in mg l-1 in which the lc50 is expected to fall. Of the collectors tested in this way sodium ethyl and potassium amyl xanthate were the most toxic, with lc50's in the range of 30-50 mg l-1. Among the frothers, xylenol (cresylic acid) was found to be the most toxic (5.6 mg l-1 >lc50 > 3.2 mg l-1) while polypropylene glycol was least toxic (lc50 > 1000 mg l-1).

The long-term tests using potassium ethyl, sodium isopropyl, sodium ethyl, and potassium amyl xanthate indicated that in the flow-through system, the toxicity of the chemicals was in the order of 100 fold greater compared with the static bioassay results.

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Potassium amyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 4.5 mg/l (NOEC). One of the 96h-LC50 was 18 mg/l
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Reaction mass of SIBX are related compound to PAX
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
SPECIES #
(NAME): 4 Oncorhynchus mykiss (Rainbow Trout)
AGE: NR NR
LIFE STAGE: fingerling
COMMENT: 1-10 G
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
348 mg/L CaCO3
ALKALINITY: 203 mg/L CaCO3
Test temperature:
12 0C
pH:
8.6
Dissolved oxygen:
Saturated mg/l
Salinity:
SALINITY:NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F NR 70000 TO 75 000 70000 TO 75000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 96 hour(s)
STAND DUR. (D): 4 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F NR 70000 TO 75 000 70000 TO 75000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 12 NR TO NR C
pH: 8.6 NR TO NR
HARDNESS: 348 NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: 203 NR TO NR mg/L CaCO3
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: SAT NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
70 - 75 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Potassium amyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 14mg/l (NOEC). One of the 96h-LC50 was between 70 and 75 mg/l.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
70 - 80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Under the conditions of the present test Potassium amyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 14mg/l (NOEC). One of the 96h-LC50 was between 70 and 80 mg/l.
Details on results:
EFFECT RESULTS
EFFECT: Mortality
TREND: NR
RESPONSE SITE:NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

ENDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.: X
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (F): NR ( NR to NR)
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test Potassium amyl xanthate induced no mortality in Oncorhynchus mykiss (Rainbow Trout) below 14mg/l (NOEC). One of the 96h-LC50 was between 70 and 75 mg/l.
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Sodium hydroxide is both reagents used in the manufacture of Reaction mass of 2-methylpropan-1-ol and sodium O-isobutyl dithiocarbonate and sodium hydroxide . Therefore, sodium hydroxide need to be considered in the assessment of Reaction mass of 2-methylpropan-1-ol and sodium O-isobutyl dithiocarbonate and sodium hydroxide
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Poecilia reticulata
Details on test organisms:
SPECIES #
(NAME): 28 Poecilia reticulata (Guppy)
AGE: 3-4 wk
LIFE STAGE: young organism(s)
COMMENT: NR
Test type:
semi-static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
NR
Test temperature:
24 0C
pH:
>9.8 to < 10.0
Dissolved oxygen:
>= 70% Sat
Salinity:
~ 2.8 %
Nominal and measured concentrations:
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: mg/L milligram per Liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
T 196 144 TO 267 196000(144000-267000) ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: SW (Salt water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: C (Concurrent control)
EXPOSURE TYPE: R( semi-static),
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 48hour(s),
STAND DUR. (D): 2 day(s)

TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: mg/L milligram per Liter
EXP. DESIGN: PRECIPITATE FORMED AT >=180 MG/L

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
T 196 144 TO 267 196000(144000-267000) ug/L NR



WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 24 NR TO NR C
pH: NR >9.8 TO <10
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: ~ 2.8 NR TO NR %
ALKALINITY: NR NR TO NR NR
CONDUCTIVITY: NR NR TO NR NR
DISSOLVED OXYGEN: >= 70 NR TO NR % Sat
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
196 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (144mg/l to 267mg/l)
Details on results:
A 48 h LC50 of 196 mg/L (saltwater) has been determined for the effects of the test substance on mortality of the Poecilia reticulata (Guppy)
EFFECT RESULTS
EFFECT: Mortality
TREND: INC
RESPONSE SITE: NR
% EFFECT: NR
EFFECT MEASUREMENT:Mortality

NDPOINT
ENDPOINT: Lethal concentration to 50% of test organisms
ENDPOINT ASSIGN.:P
SIGNIFICANCE: NA
LEVEL: NA
BCF Value (T): NR ( NR to NR)
Validity criteria fulfilled:
yes
Conclusions:
A 48 h LC50 of 196 mg/L (saltwater) has been determined for the effects of the test substance on mortality of the Poecilia reticulata (Guppy)
Endpoint:
short-term toxicity to fish
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Carbon disulphide is both a reagent in the manufacture, as well as a decomposition product of xanthates. Reaction mass of SIBX readily decomposes to carbon disulphide, especially in the presence of moisture/water. Therefore, the health effects of carbon disulphide (CS2) need to be considered in the assessment of Reaction mass of SIBX.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
incubation in sealed vessels due to volatility of CS2
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
not specified
Details on test solutions:
- Stock solution were prepared fresh each day.
- Test solutions were renewed daily.
Test organisms (species):
Poecilia reticulata
Details on test organisms:
SPECIES #
(NAME): 28 Poecilia reticulata
(Guppy)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
225 mg CaCO3/L
Test temperature:
20 °C
pH:
8.1
Dissolved oxygen:
Not reported
Nominal and measured concentrations:
Not reported
Details on test conditions:
Tests were carried out in sealed vessels to prevent evaporation.
The complete compostion of the test medium is given in Van Leeuwen and Maas, Environ. Pollution 37: 105-115 (1985)
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL = 3.0 - 5.8 mg/L mg/L
Details on results:
Under the conditions of the present test the 96-h LC50 of Carbon disulphide (CS2) as a decomposition product of Sodium isobutyl xanthate in the presence of water was 4 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test the 96-h LC50 of Carbon disulphide (CS2) as a decomposition product of Sodium isobutyl xanthate in the presence of water was 4 mg/L.
Executive summary:

A semi-static test in a sealed system was carried out to determine the toxicity of CS2 as a decomposition product of Sodium isobutyl xanthate in the presence of water to the fish Poecilia reticulata. The test fulfilled the requirements of OECD Guidline 203 but was not carried out in compliance with GLP. The 96-h LC50 of CS2 for Poecilia reticulata was 4 mg/L.

Endpoint:
short-term toxicity to fish
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction:US EPA accepted QSAR method for chemicals properties assessment.
Qualifier:
according to
Guideline:
other: QSAR
Principles of method if other than guideline:
EPI Suite v 4.1/ECOSARv1.00
GLP compliance:
no
Remarks:
not applicable to QSAR models
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: Fishes species
Details on test organisms:
no further information
Test type:
other: QSAR model
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
not applicable. QSAR model
Test temperature:
not applicable. QSAR model
pH:
not applicable. QSAR model
Dissolved oxygen:
not applicable. QSAR model
Salinity:
not applicable. QSAR model
Details on test conditions:
100% Sodium isobutyl xanthate (CAS No. 25306-75-6)
SMILES : O(C(CC)C)C(S([Na]))=S
CHEM : Carbonodithioic acid, o-(2-methylpropyl) ester, sodium salt
MOL FOR: C5 H9 O1 S2 Na1
MOL WT : 172.24
Log Kow: 1.78 (used)
Wat Sol: 663 mg/L (measured)
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
207.302 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Details on results:
Using the EPIWIN ECOSAR QSAR model, a 96hr LC50 for freshwater fish of 207.302mg/l was established.
Fish, 96-hr LC50 =207.302mg/L (ppm)
Validity criteria fulfilled:
yes
Remarks:
US EPA accepted QSAR method for chemicals properties assessment.
Conclusions:
Using the EPIWIN ECOSAR QSAR model, a 96hr LC50 for freshwater fish of 207.302 mg/l was established.
Fish, 96-hr LC50 =207.302 mg/L (ppm)
Fish, 96-hr (SW) LC50 =293.065mg/L (ppm)
Fish 14-day LC50 = 208.864 mg/L (ppm)
Fish 30-day ChV = 21.281 mg/L (ppm)
Fish (SW) 30-day ChV = 26.607 mg/L (ppm)
Executive summary:

The ECOSAR QSAR model predicts that the 96hr LC50 for freshwater fish would to be 207.302 mg/l therefore that the substance is effectively not toxic to fishes.

Endpoint:
short-term toxicity to fish
Type of information:
(Q)SAR
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.
Qualifier:
according to
Guideline:
other: QSAR
Principles of method if other than guideline:
EPI Suite v 4.1/ECOSARv1.00
GLP compliance:
no
Remarks:
not applicable to QSAR models
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
other: Fishes species
Details on test organisms:
no further information
Test type:
other: QSAR model
Water media type:
saltwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
not applicable. QSAR model
Test temperature:
not applicable. QSAR model
pH:
not applicable. QSAR model
Dissolved oxygen:
not applicable. QSAR model
Salinity:
not applicable. QSAR model
Details on test conditions:
100% Sodium isobutyl xanthate (CAS No. 25306-75-6)
SMILES : O(C(CC)C)C(S([Na]))=S
CHEM : Carbonodithioic acid, o-(2-methylpropyl) ester, sodium salt
MOL FOR: C5 H9 O1 S2 Na1
MOL WT : 172.24
Log Kow: 1.78 (used)
Wat Sol: 663 mg/L (measured)
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
293.065 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Details on results:
Using the EPIWIN ECOSAR QSAR model, a 96hr LC50 for marine water fish(SW) of 293.065 mg/l was established.
Fish, 96-hr LC50 =293.065 mg/L (ppm)
Validity criteria fulfilled:
yes
Remarks:
US EPA accepted QSAR method for chemicals properties assessment.
Conclusions:
Using the EPIWIN ECOSAR QSAR model, a 96hr LC50 for marine water fish(SW) of 293.065 mg/l was established.
Fish, 96-hr LC50 =207.302 mg/L (ppm)
Fish, 96-hr (SW) LC50 =293.065mg/L (ppm)
Fish 14-day LC50 = 208.864 mg/L (ppm)
Fish 30-day ChV = 21.281 mg/L (ppm)
Fish (SW) 30-day ChV = 26.607 mg/L (ppm)
Executive summary:

The ECOSAR QSAR model predicts that the 96hr LC50 for marine water fish (SW) would to be 293.065 mg/l therefore that the substance is effectively not toxic to fishes.

Description of key information

The lowest LC50 was observed for Sodium O-isobutyl dithiocarbonate (SIBX) as a main constituent of Reaction mass of 2-methylpropan-1-ol and sodium O-isobutyl dithiocarbonate and sodium hydroxide  toxicity to Oncorhynchus mykiss (Rainbow Trout) in freshwater: 96h-LC50 is 70 mg /L
The ECOSAR QSAR model predicts that the 96hr LC50 for marine water fish (SW) would to be 293.065 mg/l therefore that the substance is effectively not toxic to fishes.

Key value for chemical safety assessment

LC50 for freshwater fish:
70 mg/L
LC50 for marine water fish:
293.065 mg/L

Additional information

The lowest LC50 was observed for Sodium O-isobutyl dithiocarbonate (SIBX) as a main constituent of Reaction mass of 2-methylpropan-1-ol and sodium O-isobutyl dithiocarbonate and sodium hydroxide toxicity to Oncorhynchus mykiss (Rainbow Trout) in freshwater: 96h-LC50 is 70 mg /L

The ECOSAR QSAR model predicts that the 96hr LC50 for marine water fish (SW) would to be 293.065 mg/l therefore that the substance is effectively not toxic to fishes.