4,4,5,5,6,6,7,7,8,8,9,9,9-tridecafluorononan-1-ol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Remarks:

Conducted according to guideline in effect at time of study conduct

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctan-1-ol

EC Number:

211-477-1

EC Name:

3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctan-1-ol

Cas Number:

647-42-7

Molecular formula:

C8H5F13O

IUPAC Name:

3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctan-1-ol

Details on test material:

- Purity: 99.7%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Replicate A, all test concentrations including the dilution water control, day 0, day 2 renewal (old and new), day 4 old.
- Sampling method: LC/MS analysis
- Sample storage conditions before analysis: refrigerated upon receipt and when not in use.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
Test solutions were prepared by adding the appropriate volume of the test material to the appropriate volume of Haskell Well Water in 4 L Erlenmeyer flasks filled to attain zero headspace (final volume approximately 4.3L) and sonicating for approximately 20 minutes, stirring for approximately 20 minutes, then repeating the sonicating and stirring for two additional cycles, for a total mixing time of approximately 2 hours. The dilution water control and all test solutions were clear and colourless with no visible precipitate.

Test organisms

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Age at study initiation: >112 days
- Feeding during test: no

ACCLIMATION PERIOD: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

- Total Alkalinity: 49 mg/L as CaCO3
- EDTA Hardness: 116 mg/L as CaCO3

Test temperature:

21.4 to 21.6°C

pH:

Dilution Water Control: 7.0 - 7.3

Dissolved oxygen:

Dilution Water Control: 5.4 - 8.5 mg/L

Salinity:

freshwater

Nominal and measured concentrations:

Nominal concentrations: 0, 1.0, 2.0, 4.0, 8.0, and 16.0 mg/L
Measured concentrations: 0, 0.751, 1.61, 3.12, 7.52 and 16.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 4-L Erlenmeyer flasks
- Renewal rate of test solution (frequency/flow rate): renewed at day 2 (48 hours)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS: Water quality parameters were determined to be within acceptable limits.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality and sublethal effect observations were made at test start, every 24 hours thereafter, and at test end.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness, which included 30 minutes of transitional light (3-125 Lux) preceding and following the 16-hour light interval.
- Light intensity: approximately 126-710 Lux

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

4.84 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% confidence limits of 3.12 to 7.52 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

3.12 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

7.52 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: none observed
- Observations on body length and weight: Fish in the dilution water control ranged from 2.2 to 2.8 cm in standard length and 0.140 to 0.304 g in wet weight, blotted dry, at test end.
- Mortality of control: none
- Mortality of mean measured test concentrations: 100% mortality at 7.52, and 16.4 mg/L

Reported statistics and error estimates:

96-hour LC50 values and confidence limits were calculated by the binomial method based on mean, measured concentrations of the test substance and mortality. The highest mean, measured concentration causing no mortality at test end and the lowest mean, measured concentration causing 100% mortality at test end were assessed by visual observation.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
96-h LC50 = 4.84 mg/L
96-h NOEC = 3.12 mg/L.
96-h LC100 = 7.52 mg/L.

Executive summary:

The acute toxicity of the test substance to unfed juvenile fathead minnow, Pimephales promelas, was determined in an unaerated, static-renewal 96-hour toxicity test. The study was conducted with 5 concentrations of the test substance and a dilution water control at a mean temperature of 23.0°C (range of 21.5-26.0°C). The test solutions were renewed once after 48 hours. Two replicate test chambers were used per test concentration with 5 fish in each chamber (10 fish per concentration). Exposure of fathead minnow to mean, measured test substance concentrations of 0.751, 1.61, 3.12, 7.52, and 16.4 mg/L resulted in 0, 0, 0, 100, and 100% mortality, respectively, at the end of 96 hours. No mortality or sublethal effects were seen in the dilution water control fish. Mean, measured concentrations ranged from 75 to 103% of the nominal concentrations. The highest mean, measured concentration causing no mortality at test end was 3.12 mg/L. The lowest mean, measured concentration causing 100% mortality at test end was 7.52 mg/L. Mean, measured concentrations of the test substance were used for calculation of LC50 values. The 96-hour LC50, based on the mean, measured concentrations of the test substance and mortality, was 4.84 mg/L.