Sodium 4-chloro-3-[4-[[5-chloro-2-(2-chlorophenoxy)phenyl]azo]-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl]benzenesulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Test GLP compliant and conducted according to internationally accepted testing guidelines.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Principles of method if other than guideline:

Internal method SAV: 14 000 02

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

All test medium samples for the analytical determinations were taken from the approximate center of the test vessels ( after mixing the medium ) and kept at-18 °C to -25 °C until analyses.The concentrations of the test substance at the beginning and at the end of the test were analysed by the PE Analytical Services K, Textile Dyes Division.

Details on test solutions:

0.5 g test substance were mixed with 200 ml test medium and filled up to 500 ml with test medium.The stock solution was homogenized for 10 minutes by ultrasonic treatment.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM- Source: The Daphnia were bred in the laboratories of the Product Ecology TF, Textile Dyes Division under standardised conditions.- Breeding : Cultures of Daphnia magna are held in glass vessels containing aprox. 3.0 litre of reconstituted water at 18 - 22 °C. The water was renewed partially three times per week. The Daphnia were feed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a TETRAMIN-extract in such quantities that the food was consumed within 24h.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

214 mg/l CaCO3

Test temperature:

21 ± 1°C

pH:

7.8 - 8.2

Nominal and measured concentrations:

The nominal test concentrations were chosen as: 21, 45, 100 and 200 mg/I. In the test vessels non alteration of the test substance was observed.

Details on test conditions:

TEST SYSTEM- Test vessel: 250 ml beaker- Type (delete if not applicable): closed- Material: 100 - 200 ml test medium- Aeration: no- No. of organisms per vessel: 10- No. of vessels per concentration (replicates): 2- No. of vessels per control: 1TEST MEDIUM / WATER PARAMETERS- Source/preparation of dilution water:NaHC03 65 mg/lMgS04 X 7 H20 123.3 mg/lKCl 5.8 mg/lCaCI2 X 2 H20 294 mg/lNa2SiCO3 4.3 mg/lNaN03 294 mg/lKH2P04 143 µg/lK2HP04 184 µg/l- Alkalinity: 0.8 mmol/I- Ca/mg ratio: 4 : 1- Na/K ratio: 10 : 1OTHER TEST CONDITIONS- Adjustment of pH: no- Photoperiod: 16 hours light / 8 hours dark

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

The EC50 - value are calculated with the " Probit Model ".

No immobilization and abnormal behaviour of the Daphny was observed in the control.

Validity criteria fulfilled:

yes

Conclusions:

EC50 (48h) > 18 mg/l

Executive summary:

The substance has been tested for acute toxicity on aquatic invertebrates. This study was performed in acordance with Good Laboratory Practice (GLP) principles in Switzerland. The EC50 value at 48(h) is > 18 mg/l and consequently the substance is to be considered dangerous for the environment,

Description of key information

EC50 > 18 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

18 mg/L

Additional information

The short term toxicity to aquatic invertebrates was determined using the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).

Based on the test result, the tested substance showed some toxic effect on Daphnia.