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EC number: 812-877-4 | CAS number: 1228350-17-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was selected as the key study because the information provided for the hazard endpoint is sufficient for the purpose of classification and labelling and/or risk assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Remarks:
- The study was conducted according to guideline in effect at time of study conduct.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- Remarks:
- The study was conducted according to guideline in effect at time of study conduct.
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl methacrylate
- EC Number:
- 218-407-9
- EC Name:
- 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl methacrylate
- Cas Number:
- 2144-53-8
- Molecular formula:
- C12H9F13O2
- IUPAC Name:
- 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl methacrylate
- Details on test material:
- - Purity: not reported
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Nominal test concentration was 0.05 mg/L. Analysis of the test preparations at 0 and 48 hours showed measured test concentrations to range from 0.020 to 0.023 mg/L (fresh media) and 0.0097 to 0.019 mg/L (old media).
- Sampling method: Samples of the solvent control (replicates R1 - R4 pooled) and the 0.050 mg/L test group (replicates R1 - R2 and R3 - R4 pooled) were taken at 0 hours (fresh media), 24 hours (old and fresh media) and 48 hours (old media).
- Sample storage conditions before analysis: Further samples were taken and stored at approximately - 20°C for further analysis if necessary.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: An amount of test material (250 mg) was dissolved in dimethylformamide and the volume adjusted to 25 mL to give a 250 mg/25 mL solvent stock solution. An aliquot (500 µL of this solvent stock solution was added to reconstituted water and the volume adjusted to 5 litres to give a 1.0 mg/L stock solution. This stock solution was then centrifuged at 40000 g for 30 minutes to remove any dispersed test material and give a nominal test concentration of 0.050 mg/L. The solvent stock solution was prepared by dissolving 200 µL of dimethylformamide in to 2 litres of reconstituted water. Each stock solution and prepared concentration was inverted several times to ensure adequate mixing and homogeneity.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia
- Source: in-house laboratory cultures.
- Age at study initiation: less than 24 hours old
- Feeding during test: no
ACCLIMATION
- Type and amount of food: Each culture was fed daily with a suspension of algae (Chlorella sp.).
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- approximate theoretical total hardness of 250 mg/L as CaC03.
- Test temperature:
- 20.1 °C to 21.1 ºC
- pH:
- 7.8 ± 0.2
- Salinity:
- fresh water
- Nominal and measured concentrations:
- Nominal concentration of 0.050 mg/L
Measured test concentrations range from 0.020 to 0.023 mg/L (fresh media) and 0.0097 to 0.019 mg/L (old media) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml glass jars
- Type: closed
- Material, size, headspace, fill volume: approximately 200 ml of test preparation
- Aeration: no
- Renewal rate of test solution: renewal at 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
a) CaCl2.2H2O 11.76 g/L
b) MgS04.7H20 4.93 g/L
c) NaHC03 2.59 g/L
d) KCI 0.23 g/L
An aliquot (25 mL) of each of solutions a-d was added to each litre (final volume) of deionised water. The reconstituted water had a pH of 7.8 ± 0.2 adjusted (if necessary) with NaOH or HCI and was aerated until the dissolved oxygen concentration was approximately air-saturation value.
OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: 24 hr dark
EFFECT PARAMETERS MEASURED: Any immobilisation or adverse reactions to exposure were recorded at 24 and 48 hours after the start of exposure. The criterion of effect used was that Daphnia were considered to be immobilised if they were unable to swim for approximately 15 seconds after gentle agitation.
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: In the initial range-finding test Daphnia were exposed to a series of nominal test concentrations of 0.0065 and 0.065 mg/L.
- Results used to determine the conditions for the definitive study: yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.017 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.017 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- The time-weighted mean measured test concentrations of the test media using the mean of the measured test concentrations of replicates Rl1 and R2 pooled and replicates R3 and R4 pooled were calculated.
An estimate of the EC50 values was given by inspection of the immobilisation data. The ECso values and associated confidence limits at 24 hours were calculated by the probit method (Finney 1971) using the ToxCalc computer software package (ToxCalc 1999) and at 48 hours by the trimmed Spearman-Karber method (Hamilton et at 1977) using the ToxCalc computer software package (ToxCalc 1999).
In order to give a worst case analysis of the data it was considered justifiable to calculate the results based on the time-weighted mean measured test concentration of the test media. The time-weighted mean measured test concentration was calculated to be 0.017 mg/L.
Any other information on results incl. tables
Range-finding Test: No immobilisation was observed at the test concentrations of 0.0065 and 0.065 mg/l throughout the duration of the test.
Definitive Test: No immobilisation was observed at the single concentration tested.
In order to give a worst case analysis of the data it was considered justifiable to calculate the results based on the time-weighted mean measured test concentration of the test media. The time-weighted mean measured test concentration was calculated to be 0.017 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study and the conclusions which are drawn from it fulfil the quality criteria (validity, reliability, repeatability).
48-hour EC50 > 0.017 mg/L - Executive summary:
The toxicity of the test substance to Daphnia magna, was determined in a 48-hour, semi-static toxicity test. This study was conducted as a limit test at the concentration around solubility of the test item in dilution water to confirm the effect on the test organisms.
This experimental design conforms to a "Limit test" to confirm that at the highest attainable test concentration of 0.050 mg/l, no immobilisation or adverse reactions to exposure were observed. In order to give a worst case analysis of the data it was considered justifiable to calculate the results based on the time-weighted mean measured test concentration of the test media. The time-weighted mean measured test concentration was calculated to be 0.017 mg/L.
The 48-hour EC50 was determined to be greater than 0.017 mg/L
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