1H-Pyrazole-3-carbonitrile, 4,4'-dithiobis[5-amino-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-08-30 - 1995-09-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

5-amino-4-({5-amino-3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazol-4-yl}disulfanyl)-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazole-3-carbonitrile

EC Number:

603-434-4

Cas Number:

130755-46-3

Molecular formula:

C22 H8 Cl4 F6 N8 S2

IUPAC Name:

5-amino-4-({5-amino-3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazol-4-yl}disulfanyl)-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazole-3-carbonitrile

Details on test material:

- Name of test material (as cited in study report): MB 046307
- Physical state: yellow powder
- Analytical purity: 972 g/kg
- Lot No.: 95-4023
- Storage condition of test material: Test substance was stored in a stoppered container at room temperature, in the dark

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Sampling method: Samples of test solutions (50 ml) were taken for analysis from each bulk preparation at test initiation and from each concentration level (pooled replicates) at the termination of each 24-h cycle. One sample was analyzed.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Stock solution: Dissolution of 30 mg test substance in 2 ml dimethyl formamide (DMF). Stock solutions of the four lower test substance concentrations were prepared by subsequent dilution with DMF.
- Test solutions: Dilution of the stock solutions
- Chemical name of vehicle: dimethyl formamide (DMF)
- Concentration of vehicle in test medium (final test solution): 0.1 ml/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: clone 5 from IRCHA
- Source: Laboratory cultures, Aquatic Ecotoxicology facilities, Rhône Poulenc Agrochimie, Sophia Antipolis, France
- Age at study initiation (mean and range, SD):< 24 hours
- Feeding during test: no

CULTURE CONDITIONS
- Culture conditions: same as test
- Culture medium: dilution water
- Aeration: continuously (air bubbling)
- Type and amount of food: Daphnids were fed a mixture of a fish food suspension, meat extract, yeast suspension, seaweed extract and unicellular green algae (Chlorella vulgaris).
- Feeding frequency: Three times per week

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

ca. 170 mg/L CaCO3

Test temperature:

20.7 - 21.7 °C

pH:

7.8 - 8.0

Dissolved oxygen:

8.2 - 8.5 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 0.06, 0.14, 0.31, 0.68, 1.5 mg/L
Mean measured concentrations: < 0.01, 0.04, 0.08, 0.21, 0.48, 1.25 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: covered with plastic sheets to prevent evaporation
- Material, total volume, fill volume: glass, 250 ml, 200 ml
- Renewal rate of test solution (frequency): Each test solution was completely renewed after 24 hours and substituted by a freshly prepared test so!utlon giving two 24-hour cycles of exposure for each treatment level.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water consists of reconstituted water (80% DSW (Dutch Standard Water); 20% LC-oligo medium. Dilution water is prepared from municipal water (reverse-osmosed, de-ionized and filtered through activated charcoal and 0.22 µm filters).
- Conductivity: 562 - 573 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0, 24 h

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours light/8 hours dark
- Light intensity: 400 - 1500 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilization, behavioral abnormalities

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study: yes
- Test concentrations: control, solvent control, 0.02, 0.2, 2 mg/L
- Results used to determine the conditions for the definitive study: No immobilization was observed in the control group. Immobilization percentages of 10, 20, 60 and 90% were recorded for the daphnids exposed to the solvent, 0.02, 0.2 and 2 mg/l, respectively.

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 0.14 - 0.28 mg/L

Details on results:

- Immobilization of control: none