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EC number: 603-434-4 | CAS number: 130755-46-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995-08-29 - 1995-09-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-guideline study with analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 5-amino-4-({5-amino-3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazol-4-yl}disulfanyl)-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazole-3-carbonitrile
- EC Number:
- 603-434-4
- Cas Number:
- 130755-46-3
- Molecular formula:
- C22 H8 Cl4 F6 N8 S2
- IUPAC Name:
- 5-amino-4-({5-amino-3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazol-4-yl}disulfanyl)-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-1H-pyrazole-3-carbonitrile
- Details on test material:
- - Name of test material (as cited in study report): MB 046307
- Physical state: yellow powder
- Analytical purity: 972 g/kg
- Lot No.: 95-4023
- Storage condition of test material: in the dark at room temperature (ca. 20 °C)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples of test solutions were taken for analysis from each bulk preparation at test initiation (0 hour) and from pooled test solutions at test termination (72 hours). Each sample volume was 50 mI and was collected from the approximate midpoint of the container. Samples were taken in duplicate. AIl samples were prepared immediately after sampling and either analyzed within 24 hours after preparation or appropriately preserved and stored until analysis.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Stock solution: A stock solution of the highest test substance concentration was prepared by dissolution of 40 mg of MB 046307 in 2 ml of DMF (Dimethyl formamide). Stock solutions of the four lowest test substance concentrations were then prepared by serial dilution of the first stock solution in DMF. Bulk volumes for each of the test solutions were prepared by diluting with dilution water. Each bulk preparation was submitted to magnetic agitation for homogenization. Algal inoculum was subsequently added to each bulk preparation to give final volumes of approximately 400 mI per treatment level and solvent control and 700 ml for the dilution water control.
- Test solutions: Treatment replicate vessels were filled from bulk preparation to 100 ml each.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Dimethyl formamide (DMF)
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Collection of the University of Göttingen, Germany. Cultured by the laboratory of the Aquatic Ecotoxicity testing facilities, Rhône-Poulenc Agrochimie, Centre de Recherche, Sophia Antipolis, France.
- Age of inoculum (at test initiation): The algal cells used for the test cultures were taken from a preculture which was initiated 96 hours prior to the test under the same conditions as in the test.
CULTIVATION
- Culturing media and conditions: Same as test. The culturing medum was a diluted nutrient concentrate. Algae were cultured in glass vessels of 500 ml containing ca. 300 ml of culture solution. The vessels were fitted with stainless steel caps. The cultures were maintained in an air conditioned culture room at a temperature of 23°C ± 2°C and transfers were made regularly into fresh sterile media.
- Photoperiod: 16 hours light and 8 hours darkness
- Light inensity: Approximately 1 000 Lux
- Agitation: Manual agitation at least once per working day
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- Study initiation: 24.4 - 24.8 °C
Study end: 22.9 - 23.2 °C - pH:
- Study initation: 7.4 - 7.5
Study end: 7.5 - 8.1 - Nominal and measured concentrations:
- Nominal test concentrations: control, 0.09, 0.19, 0.41, 0.91, 2.0 mg/L
Mean measured concentrations: < 0.01, 0.04, 0.12, 0.28, 0.65, 1.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, fill volume: Erlenmayer flasks fitted with stainless steel caps which permitted gas exchange. Each test vessel contained 100 ml of test solution.
- Initial cells density: 17500 - 22500 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0, 72 h
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: artificial illumination, 8000 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Cell counts were performed using a Malassez haemocytometer and a microscope
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Range finding study
- Test concentrations: 0.02, 0.2 and 2.0 mg/l, a solvent control and a dilution water control (two replicates for each group).
- Results used to determine the conditions for the definitive study: No significant growth inhibition percentage was observed at any of the test concentrations showing the EC50 values of MB 046307 to be above the visual limit of aqueous solubility.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: the loading of 1.5 mg/L is in excess of the visual limit of aqueous solubility
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1.5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: the loading of 1.5 mg/L is in excess of the visual limit of aqueous solubility
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: Concentrations over 0.41 mg/L were above the visual limit of aqueous solubility. This was defined by the presence of undissolved particles in these test solutions at test initiation.
- Effect concentrations exceeding solubility of substance in test medium: yes
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