Reaction mass of (2S,6S)-2,6-Dimethylmorpholine and (2R,6R)-2,6-Dimethylmorpholine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,6-dimethylmorpholine

EC Number:

205-509-3

EC Name:

2,6-dimethylmorpholine

Cas Number:

141-91-3

IUPAC Name:

2,6-dimethylmorpholine

Details on test material:

- Name of test material (as cited in study report): 2,6 Dimethylmorpholin (cis/trans isomere mixture)
- Analytical purity: 98.6% (75.3% cis, 23.3% trans)
- Impurities (identity and concentrations): 1.2% water; 0.2% unknown impurities

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Concentration of the test substance in the stock solution: 500 mg/L

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation: 2- 24 h
- Feeding during test: none

HOUSING
- Culture conditions:
- Temperature: 19 - 21 °C
- Photoperiod: day:night rhythm 16:8 h
- Intensity: ca. 5 µE/m*m*s at 400 -750 nm
- Feeding: green algae culture daily, yeast after water changing

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.7 ± 0.5 mM

Test temperature:

19 - 21 °C

pH:

7.9 - 9.5

Dissolved oxygen:

8.31 - 9.04 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 7.81, 15.6, 31.2, 62.5, 125, 250, 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material: centrifuge tubes with flat bottom
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 daphnids/ ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water not chlorinated, char coal filtered, aerated with oil-free air
- Alkalinity: 0.8 ± 0.1 mM
- Ca/mg ratio: 4:1
- Conductivity: 550 - 650 µS/cm
- Intervals of water quality measurement: 0, 48 hours

OTHER TEST CONDITIONS
- Photoperiod: diffuse light

EFFECT PARAMETERS MEASURED: mobility after 3, 6, 24, 48 hours

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: data refer to an unneutralised test sample

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

187.35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: data refer to an unneutralised test sample

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: data refer to an unneutralised test sample

Details on results:

Effect concentrations after 24 h:
EC0 = 125 mg/L
EC50 = 259.13 mg/L
EC100 = 500 mg/L