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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 May - 25 Oct 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report Date:
- 2013
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- (Adopted:23 March 2006, Annex 5 corrected: 28 July 2011)
- Deviations:
- no
- Qualifier:
- according to
- Guideline:
- other: Testing Methods for New Chemical Substances; 2011.03.31, No. 0331-07, Ministry of Health, Labor and Welfare; 2011.03.29 No 5, Ministry of Economy, Trade and Industry; No. 110331009, Ministry of Environment, Japan; IV. Algae growth inhibition test.
- Deviations:
- no
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): [trade name given]
- Physical state: white crystalline powder
- Analytical purity: 100%
- Lot/batch No.: KNC110310
- Expiration date: 14 Mar 2014
- Stability under test conditions: stable for 3 years in ambient temperature
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 100 mg/L
- Sampling method: An aliquot was taken from the remaining unused test solution of each test group at initiation and termination of the test. The samples were taken from mid-depth of each replicate without agitation.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 0.0802 g aliquot of the test substance was weighed. A stock solution of concentration 100 mg/L was prepared to make a volume to 800 mL with the medium.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: ATCC22662
- Source (laboratory, culture collection): in-house culture originates from American Type Culture Collection (received 01 Aug 1996)
- Method of cultivation: aseptic culture
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.7 - 23.5 °C
- pH:
- 7.6 - 7.7 at the begining
8.1 - 8.3 at the end - Nominal and measured concentrations:
- nominal: 0, 100 mg/L
measured: < LOQ, 100 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flasks with an air-permeable silicone stopper
- Volume of test solution: 100 mL/vessel
- Aeration: continuously shaken at 100 rpm
- Initial cells density: 1 x 10^4 cells/mL
- Control end cells density: 1.77 x 10^6 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Standard medium used: yes (OECD 201 recommended test medium)
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous fluorescent illumination
- Light intensity and quality: cool-white light, 400-700 nm, 60-120 µE/m²/s (± 15%) at the surface of the cultures
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: electronic particle counter after 24, 48 and 72 h
- Appearance: once daily
- Morphological changes: microscopic observation at the end of the test
- Range finding study
- Test concentrations: 0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: 3.7% inhibition of growth rate at 10 mg/L, 2.3% inhibition of growth rate at 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): not reported
- Unusual cell shape: not reported
- Colour differences: not reported
- Flocculation: not reported
- Adherence to test vessels: not reported
- Aggregation of algal cells: not reported - Reported statistics and error estimates:
- Student t-test
Any other information on results incl. tables
Table 1: Growth inhibition rates
Nominal concentration [mg/L] | Vessel No. | Growth rate 0 - 72 h [day -1] | Percent inhibition [%] |
Medium control | 1 | 1.7337 | - |
2 | 1.7124 | - | |
3 | 1.7293 | - | |
4 | 1.7244 | - | |
5 | 1.7355 | - | |
6 | 1.7213 | - | |
Mean | 1.7261 | - | |
SD | 0.0086 | - | |
CV | 0.5 | - | |
100 | 1 | 1.6741 | 3.0 |
2 | 1.7172 | 0.5 | |
3 | 1.7003 | 1.5 | |
4 | 1.6884 | 2.2 | |
5 | 1.7179 | 0.5 | |
6 | 1.7120 | 0.8 | |
Mean | 1.7016 | 1.7* | |
SD | 0.0176 | - |
* Statistically different
It is considered that the significant difference at 100 mg/L was not the results of the test substance treatment but of incidentally small relative variation between replicates of each test group. Although statistical significance was detected, the difference in the growth inhibition rate between 100 mg/L and control group was very small and was not considered biologically relevant. The statistical significance should be attributed not to the treatment of test substance but to the incidentally small relative variation of the values between replicates in each test group."
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