2,2'-(1,4-phenylene)bis[4-[(4-methoxyphenyl)methylene]oxazol-5(4H)-one]

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1977

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: early study, no GLP; short report, females only

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Remarks:

pre-dates GLP regulation

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-(1,4-phenylene)bis[4-[(4-methoxyphenyl)methylene]oxazol-5(4H)-one]

EC Number:

257-055-0

EC Name:

2,2'-(1,4-phenylene)bis[4-[(4-methoxyphenyl)methylene]oxazol-5(4H)-one]

Cas Number:

51202-86-9

Molecular formula:

C28H20N2O6

IUPAC Name:

2,2'-(1,4-phenylene)bis[4-(4-methoxybenzylidene)-1,3-oxazol-5(4H)-one]

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: sesame oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 25 %

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: no data
- Frequency of weighing: weekly
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

none

Clinical signs:

other: balance disorder and prone position. feces showed a yellow discoloration

Gross pathology:

no effects

Other findings:

none

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The median lethal dose (LD50) after single oral administration to female rats is greater than 5000 mg/kg body weight.

Executive summary:

The test substance, a yellow powder, was administered by oral gavage formulated as a 25% suspension in sesame oil at a single dose of 5.000 mg/kg body weight to 10 female SPF-Wistar-rats (strain: Hoe:WISKf (SPF 71) weighing 190-210g (average weight 201g).

The test system with female rats was chosen, because no differences in sensitivity between sexes were found in preliminary studies. Food was withheld from 16 h before to 2 h after dosing.

The observation period following treatment lasted for 14 days. During this time the animals were weighed weekly, and fed with laboratory diet ALTROMIN 1324 (Altromin GmbH, Lage/Lippe) and tap water ad libitum. Animals were kept in plastic cages on woodshaves.

At the end of the observation period the animals were sacrificed after narcotisation, dissected and examined for macroscopically visible changes.

The study was performed between 1 September 1977 and 16 September 1977.

Results:

None of the 10 rats died after dosing 5000 mg/kg body weight. After administration the animals showed balance disorder and prone position. The weight gains during the observation period were normal in all the rats. After administration the excreted feces showed a yellow discoloration. The animals killed at the end of the observation period showed no macroscopically visible changes.

Due to the experimental design an exact determination of the LD 50 was not possible. However the median lethal dose (LD50) after single oral administration to female rats is definitely greater than 5000 mg/kg body weight.