Tri-alkyl Quat

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP and Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

1987

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Test material form:

other: aqueous solution

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc., Michigan, USA
- Weight at study initiation: 205-262 g

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

no vehicle used

Doses:

0, 169, 205, 248, 300 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 1, 2.5 and 4 hours after administration and once daily thereafter
- Necropsy of survivors performed: yes

Statistics:

Dose-effects were evaluated using the method developed by Litchfield and Wilcoxon.

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

207 mg/kg bw

Based on:

act. ingr.

95% CL:

197 - 216

Remarks on result:

other: Females were more sensitive than males.

Mortality:

In the 205 mg/kg bw dose group, one male and 2 females died, in the 248 mg/kg bw dose group 2 males and 5 females and in the highest dose group all animals died.

Clinical signs:

other: Diarrhoe was only observed once in the lowest dose group and occurred more frequently in the higher dose groups. Brown/red staining around the mouth was predominantly observed in the 248 and 300 mg/kg bw test groups, as well as respiratory rates, ataxia a

Gross pathology:

At necropsy, enlarged and reddened adrenal glands, meningeal haemorrhage, dark reddening of the cortico-medullary junctions in the kidneys, reddened and distended stomachs, reddened intestines, dark purple colouring of the livers and in one case a dark reddening of the lungs were found from the 205 mg/kg bw dose groups onwards.