tris(branched-alkyl) borate

Endpoint summary

Description of key information

Toxicity to earthworm

The highest concentration causing no mortality 7 d-LC0 was 10.0 mg/kg dry weight soil. 7 d-LC50 was 57.0 mg/kg dry weight soil, and the 95% confidence interval was 47.5 to 68.5 mg/kg dry weight soil. The highest concentration causing no mortality 14 d-LC0 was 10.0 mg/kg dry weight soil.14-d LC50 was 30.0 mg/kg dry weight soil, and the 95% confidence interval was 24.9 to 36.1 mg/kg dry weight soil (The guidelines for the testing of chemicals" (HJ/T 153-2004) and "The Guidelines for the Testing of Chemicals-Effects on Biotic Systems" (the 2nd edition. Beijing: China Environment Press. 2013 plus OECD 207 and EU Method C.8 ).

Toxicity to terrestrial plants

Treatment-related effects on plant germination were reported at 1000 mg a.i./kg dry soil for all the species tested and at 333.33 mg a.i./kg dry soil for oat. The study was considered valid as the validation criteria were met and no visible treatment-related effects were observed in the controls.

 

The LC25 values for germination were estimated to be > 1000 mg a.i./kg dry soil for carrot and 730 and 496 mg a.i./kg dry soil for tomato and oat respectively. The LC50 values for germination were estimated to be > 1000 mg a.i./kg dry soil for carrot and tomato and 654 mg a.i./kg dry soil for oat. The NOEC values were 1000 mg a.i./kg dry soil for carrot and 333.33 mg a.i./kg dry soil for tomato and oat.

 

There was 100 % survival of carrot and tomato at all rates of application up to and including 333.33 mg a.i./kg dry soil. Survival rates of 73.7, 100 and 83.3 % of germinated seedlings were recorded for carrot, tomato and oat respectively at 1000 mg a.i./kg dry soil and 92.1 % for oat at 333.33 mg a.i./kg dry soil.

 

The EC25 values for height were estimated to be 409.80, 429.90 and 304.95 mg a.i./kg dry soil for carrot, tomato and oat respectively. The EC50 values for height were estimated to be 734.90, 797.47 and 334.19 mg a.i./kg dry soil for carrot, tomato and oat respectively. The NOEC values for effects on height were 111.11 mg a.i./kg dry soil for all species.

 

The EC25 values for dry weight were estimated to be 251.62, 354.63 and 166.01 mg a.i./kg dry soil for carrot, tomato and oat respectively. The EC50 values for dry weight were estimated to be 445.67, 497.91 and 240.60 mg a.i./kg dry soil for carrot, tomato and oat respectively. The NOEC values for effects on dry weight were 111.11 mg a.i./kg dry soil for all species (OECD 208).

Additional information

Toxicity to earthworm

The key study was conducted to determine the acute toxicity of test substances to earthworm according to "The guidelines for the testing of chemicals" (HJ/T 153-2004) and "The Guidelines for the Testing of Chemicals-Effects on Biotic Systems" (the 2nd edition. Beijing: China Environment Press. 2013); with reference to Procedure 207 of the ' Guidelines for Testing of Chemicals' of the OECD "Earthworm, Acute Toxicity Tests" (1984) etc.

 

During this test, the artificial soils was used with the moisture content of the test medium controlled in range of 26% to 35% with pH from 5.65 to 6.47. The test conditions were maintained as follows: temperature of 20.6 °C to 22.3 °C, humidity of 80 % to 85 % and continuous light with intensity of 400 lux to 800 lux. Based on the result of range-finding test and definitive test, the 7 day and 14 day LC50 values were both determined for the test substance in the artificial soil

 

The highest concentration causing no mortality 7 d-LC0 was 10.0 mg/kg dry weight soil. 7 d-LC50 was 57.0 mg/kg dry weight soil, and the 95% confidence interval was 47.5 to 68.5 mg/kg dry weight soil. The highest concentration causing no mortality 14 d-LC0 was 10.0 mg/kg dry weight soil.14-d LC50 was 30.0 mg/kg dry weight soil, and the 95% confidence interval was 24.9 to 36.1 mg/kg dry weight soil.