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Environmental fate & pathways

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A study was conducted to determine the ready biodegradability of the read across substance (2-Propenoic acid, 2-methyl-, 2-hydroxyethyl ester, reaction products with phosphorus oxide (P2O5)) according to OECD Guideline 301B, EU Method C.4-C and ISO 9439, in compliance with GLP. The substance was tested in duplicate at the concentration of 12 mg TOC/L (corresponding to 31 mg substance/L) for a duration of 28 d. Test and reference substances (positive control: sodium acetate and toxicity control; test substance plus sodium acetate) were added to the bottles containing the microbial organisms and mineral components. The amount of CO2produced was determined by titration. Titrations were made every second or third day during the first 10 d, and thereafter every fifth day until Day 28, for the inoculum blank and test suspension. Titrations for the positive and toxicity control were made over a period of 14 d. All validity criteria were met and the reference substance reached the level for ready biodegradability by 14 d. No toxicity of the test substance was observed in the toxicity control. The relative biodegradation values calculated from the measurements performed during the test period revealed 22 and 18% biodegradation of the test substance (based on ThCO2) for the duplicate bottles tested. Under the study conditions, the substance was considered as 'not readily biodegradable' in a CO2 evolution test (Desmares-Koopmans MJE, 2015).

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