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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 May, 1998 to 20 May,1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
None
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
None
GLP compliance:
yes
Specific details on test material used for the study:
Test Substance: FAT 20202/F
Batch-No.:EN001911.F7
Purity: ca. 58 %
Appearance: red powder
Solubility: 50g/l
Storage: room temperature
Expiration date: 03/2003


Analytical monitoring:
no
Details on sampling:
None
Vehicle:
yes
Details on test solutions:
dechlorinated tap water
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
The test organism was the species zebra-fish (Brachydanio rerio) supplied by Charles River Aquatics, Someren, Netherlands. The quality of the fish was checked with an acclimatization time of 61 days in dechlorinated tap water.
For each concentration, 7 fish were used. The fish were not fed during the test.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Remarks on exposure duration:
None
Post exposure observation period:
None
Hardness:
142 mg/l CaC03
Test temperature:
23 °C
pH:
8.5
Dissolved oxygen:
99 %
Salinity:
None
Conductivity:
None
Nominal and measured concentrations:
0.02, 0.04, 0.08, 0.18, 0.40 and 0.88 mg/L
Details on test conditions:
Water:test medium
PH: 7.9-8.4
o2: 95-96 %
Test duration: 96 h
Light: daylight
Apparatus:
static test in 5 litre glass aquaria with a test volume of 3 litre, slowly aerated
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
0.08 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
0.13 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
0.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
None
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None
Sublethal observations / clinical signs:

Table: Summary of the LC values

    LC Values           Test Duration
 24 hrs  48 hrs  72 hrs  96 hrs
 NOEC  0.08  0.08  0.08  0.08
 LC0  0.08  0.08  0.08  0.08
 LC50  0.24  0.15  0.13  0.13
 95% Confidence limit  0.19 - 0.30  0.11 - 0.20  0.11 - 0.17  0.11 - 0.17
 LC 100  0.40  0.40  0.40  0.40

Determination of the LC-values

FAT 20202/F was tested in nominal concentrations of 0.02, 0.04, 0.08, 0.18, 0.40 and 0.88 mg test substance/L. Additionally a blank control without test substance was tested.

In the test concentrations no alteration of the test substance in the test media was observed during the test.

In the control and in the test concentration up to 0.08 mg/1 no mortalities and no abnormal behaviour of the test fish was observed over the test period. Therefore, the

NOEC and the LC0 value was determined to be 0.08 mg/1. The LC100 value was determined to be 0.40 mg/L.

The reported results are related to the nominal values of the test concentrations of the test article.

The LC50 value after 96 hours was calculated to be 0.13 mg/L.

Table: Mortalities of fish

Nominalcone.

Mortalities(numberofdeadfish)

mg/L

24h

48 h

72h

96 h

Blank

0

0

0

0

 0.02

 0

0

0

   0

 0.04

 0

0

0

  0

0.08

0

0

0

0

 0.18

 I

5

6

   6

0.40

7

7

7

7

0.88

7

--

--

--

Validity criteria fulfilled:
yes
Conclusions:
The LC50 of FAT 20202/F in this fish acute toxicity test was determined to be 0.13 mg/L.
Executive summary:

An acute fish toxicity test was performed with Zebra-fish ( Brachydanio rerio). Groups of fish were exposed at the test concentrations of 0.02, 0.04, 0.08, 0.18, 0.40 and 0.88 mg/L. In the test concentrations no alteration of the test substance in the test media was observed during the test. In the control and in the test concentration up to 0.08 mg/L, no mortalities and no abnormal behaviour of the test fish was observed over the test period. Therefore, the NOEC and the LC0 value was determined to be 0.08 mg/L. The LC100 value was determined to be 0.40 mg/L. The reported results are related to the nominal values of the test concentrations of the test article. The LC50 value after 96 hours was calculated to be 0.13 mg/L.

Description of key information

The LC50 of Acid Red 407 (FAT 20202/F) was determined to be 0.13 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.13 mg/L

Additional information

An acute fish toxicity test was performed with Zebra-fish (Brachydanio rerio). Groups of fish were exposed at the test concentrations of 0.02, 0.04, 0.08, 0.18, 0.40 and 0.88 mg/L. In the test concentrations no alteration of the test substance in the test media was observed during the test. In the control and in the test concentration up to 0.08 mg/l, no mortalities and no abnormal behaviour of the test fish was observed over the test period. Therefore, the NOEC and the LC0 value was determined to be 0.08 mg/l. The LC100 value was determined to be 0.40 mg/l. The reported results are related to the nominal values of the test concentrations of the test article. The LC50 value after 96 hours was calculated to be 0.13 mg/l. Several other supporting studies were also performed with following results:


FAT 20202/D when exposed to groups of zebra fish using methodology similar to OECD Guideline 203, was determined to have 96h LC50 of 1.5 mg/l.


FAT 20202/C when exposed to groups of Red Killi fish using methodology similar to OECD Guideline 203, was determined to have 48h LC50 of 1 mg/l.


FAT 20202/C when exposed to groups of Trout fish using methodology similar to OECD Guideline 203, was determined to have 48h LC50 of 0.55 mg/l.


FAT 20202/B when exposed to groups of Trout fish using methodology similar to OECD Guideline 203, was determined to have 48h LC50 of 0.55 mg/l.


FAT 20202/A when exposed to groups of Trout fish using methodology similar to OECD Guideline 203, was determined to have 48h LC50 of 0.63 mg/l.