Chromate(2-), [4-[(5-chloro-2-hydroxy-3-nitrophenyl)azo]-2,4-dihydro-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[[1-(3-chlorophenyl)-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-4-yl]azo]-4-hydroxy-5-nitrobenzenesulfonato(3-)]-, disodium

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Justification for type of information:

None

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Principles of method if other than guideline:

None

GLP compliance:

no

Specific details on test material used for the study:

Test material: FAT 20202/D

Analytical monitoring:

not specified

Details on sampling:

None

Vehicle:

not specified

Details on test solutions:

None

Test organisms (species):

other: not specified

Details on inoculum:

None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Remarks on exposure duration:

None

Post exposure observation period:

None

Hardness:

None

Test temperature:

None

pH:

None

Dissolved oxygen:

None

Salinity:

None

Conductivity:

None

Nominal and measured concentrations:

10, 32, 100 mg/l

Details on test conditions:

Refer "Any other information on materials and methods incl. tables"

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol (at 10, 32 and 100 mg/L)

Key result

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

The % inhibition observed were 19.5, 37.4 and 50.4 at 10, 32 and 100 mg/L respectively.

Results with reference substance (positive control):

The 3h IC50 for 3,5- dichlorophenol was found to be 23 mg/L.

Reported statistics and error estimates:

None

None

Validity criteria fulfilled:

no

Conclusions:

The IC50 for FAT 20202/D was found to be 100 mg/L.

Executive summary:

The toxicity of FAT 20202/D to microbial population was determined in a 3-hours respiration inhibition test according to an internal SOP. The % inhibition observed were 19.5, 37.4 and 50.4 at 10, 32 and 100 mg/L, respectively. Based on the findings of the study, the IC50 of the test substance FAT 20202/D was 100 mg/L.

Description of key information

The IC50 of the test substance FAT 20202/D is 100 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

Additional information

The toxicity of FAT 20202/D to microbial population was determined in a 3-hour respiration inhibition test. Based on the findings of the study, the IC₅₀ of the test substance FAT 2020D is 100 mg/L. In supporting studies that were performed with FAT 20202/B and FAT 20202/A, the IC50 was >300 mg/l.