Disodium 6-amino-5-[[4-[(2-bromo-1-oxoallyl)amino]-2-[(4-methyl-3-sulphonatophenyl)sulphonyl]phenyl]azo]naphthalene-2-sulphonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 February 1994 to 18 April 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: P.04.36
- Expiration date of the lot/batch: 11/98
- Appearance: Solid

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/dispersant/vehicle/test medium: ca. 2-3 g/l [in water]

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial (adaptation not specified)

Details on inoculum:

Sludge of a biological sewage treatment plant (ARA-Therwil) collected on 08.02.94.

Duration of test (contact time):

28 d

Initial conc.:

150 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Reference substance:

diethylene glycol

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Details on results:

Test substance:
The bioelimination of FAT - 40066/C measured as DOC after 28 days corrected by the blank control is: 0 % (average of 2 tests running in parallel) Adsorption after 3 hours: 0 %.

Results with reference substance:

The bioelimination, corrected by the blank control and measured as DOC (mg/l) is: 99.4 % 14th day.

Validity criteria fulfilled:

not specified

Interpretation of results:

not inherently biodegradable

Conclusions:

The bioelimination of FAT - 40066/C measured as DOC after 28 days is 0 %.

Executive summary:

The inherent biodegradability of FAT 40066/C was determined in a 28 days Zahn-Wellens - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance was tested in concentration of 150 mg/l DOC. The reference substance Diethylenglycol was also tested in a concentration of 150 mg/l DOC. Result: The bioelimination of the test substance FAT - 40066/C after 28 days is 0 % while the bioelimination of the reference substance after 14 days is 99.4 %. Based on the study results, the test substance is not inherently biodegradable.

Reference 2

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16 February 1994 to 10 March 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.5 (Degradation: Biochemical Oxygen Demand)

Deviations:

yes

Remarks:

Aerate over night after addition of the inoculum

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: P.04.36
- Expiration date of the lot/batch: 11/98

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility and stability of the test substance in the solvent/dispersant/vehicle/test medium: ca. 2-3 g/l in water

Oxygen conditions:

aerobic

Inoculum or test system:

other: Seeding water of an urban waste water

Duration of test (contact time):

5 d

Initial conc.:

> 6 - < 800 mg/L

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance:

other: D(+) - Glucose (Merck Nr. 8337) / L-Glutamic acid (Merck Nr. 291)

Remarks on result:

not measured/tested

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Results with reference substance:

The BOD5 of Glucose/Glutamic acid solution is 205 mg O2/I

Validity criteria fulfilled:

yes

Interpretation of results:

other: BOD has been determined

Conclusions:

The BOD5 of FAT 40066/C is 0 mg O2/g.

Executive summary:

The biochemical oxygen demand of FAT 40066/C is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. The test was carried out according to ISO 5815 Second Edition 1989-08-01 (E) corresponding to 92/96/EEC C.5. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance was tested in concentrations from about 6 to 800 mg/l. The reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 ml/I. Based on the study results, the BOD5 of FAT 40066/C is 0 mg O2/g while that of the reference substance is 205 mg O2/I.

Reference 3

Endpoint:

biodegradation in water: screening test, other

Remarks:

COD

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 February 1994 to 4 March 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: P.04.36
- Expiration date of the lot/batch: 11/98

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature
- Solubility of the test substance in the solvent/dispersant/vehicle/test medium: ca. 2-3 g/l in water

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

2 h

Parameter followed for biodegradation estimation:

other: COD

Reference substance:

other: Potassium hydrogen phthalate solution (Merck-Nr. 11769)

Remarks on result:

not measured/tested

Parameter:

COD

Value:

970 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate is 201 mg O2/I. (Validity: 200 ± 8 mg O2/l)

Validity criteria fulfilled:

yes

Interpretation of results:

other: COD has been determined

Conclusions:

The COD of FAT 40066/C is 970 mg O2/g.

Executive summary:

A predeterminated amount of FAT 40066/C dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test was carried out according to DEV DIN 38'409 - H 41-1 (1980) corresponding to 92/96/EEC C.6. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance was tested with a sample weight of 5.0 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I. Based on the study results, the COD of FAT 40066/C is 970 mg O2/g while that of the COD of the reference substance is 201 mg O2/I.

Description of key information

The test substance was determined to be not biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater

Additional information

The inherent biodegradability of FAT - 40066/C was determined in a 28-days Zahn-Wellens - Test according to the OECD Guideline for Testing of Chemicals, No. 302B, Paris 1981 and the EEC-Directive, L133 (pp. 99-105) Volume 31, May 88. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The test substance was tested in concentration of 150 mg/l DOC. The reference substance Diethylenglycol was also tested in a concentration of 150 mg/l DOC. Result: The bioelimination of the test substance FAT - 40066/C after 28 days is 0 % while the bioelimination of the reference substance after 14 days is 99.4 %. Based on the study results, the test substance is not inherently biodegradable. In a key study, the biochemical oxygen demand of FAT 40066/C is determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of microorganisms. The test was carried out according to EU method C.5. The test substance was tested in concentrations from about 6 to 800 mg/l. The reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 ml/I. Based on the study results, the BOD5 of FAT 40066/C is 0 mg O2/g. For the biochemical oxygen demand, a predeterminated amount of FAT 40066/C dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test was carried out according to DEVEU method C.6. The test substance was tested with a sample weight of 5.0 mg. The reference substance Potassium hydrogen phthalate solution was tested in a concentration of 20 ml/I. Based on the study results, the COD of FAT 40066/C is 970 mg O2/g. Consequently, the BOD5/COD quotient is calculated to be 0 indicating that no biodegradation of the test material takes place. In another supporting studies, the biochemical oxygen demand of FAT 40066/A is 58 mg O2/g while the chemical oxygen demand of FAT 40066/A is 1057 mg O2/g. Consequently, the BOD5/COD quotient is calculated to be 0.05 indicating that no biodegradation of the test material takes place.