2,2'-[cyclohexane-1,1-diylbis(4,1-phenyleneoxymethylene)]dioxirane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 25, 2016 ~ April 06, 20 16

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-[cyclohexane-1,1-diylbis(4,1-phenyleneoxymethylene)]dioxirane

Cas Number:

13446-84-9

Molecular formula:

C24H28O4

IUPAC Name:

2,2'-[cyclohexane-1,1-diylbis(4,1-phenyleneoxymethylene)]dioxirane

Test material form:

liquid: viscous

Test solutions

Vehicle:

no

Details on test solutions:

The toxicity of the test item was determined by preparing a water-accommodated fractions (WAF) of the test item. The water-accommodated fraction was applied to dilution water containing only the fraction of multi-component test item that was dissolved and/or present as a stable dispersion. The preparation method for the WAF was as follows:
0.118 g of the test item (purity: 85%) was added to a 1100 mL beaker and dilution water was added up to 1000 mL mark of the beaker to give the nominal loading rate of 100 mg/L. The prepared test solution was stirred for 48 hours using magnetic stirrer. Undissolved test item existed on the wall of the beaker because the test item was very sticky.
After 48 hours of mixing, the middle part of solution was siphoned off after settling for 30 minutes to achieve the WAF from the nominal 100 mg/L test item loading rate. This WAF solution was used for toxicity testing and the dilution water only used for the control.
Crystallizing dishes (120 mL) containing approximately 100 mL ofWAF were used for testing.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

19.7

pH:

8.0

Dissolved oxygen:

8.0

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (total fraction)

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48Iu·-ECso value (95% confidence limits) expressed in terms of the measured concentration of SEZ-250 to Daphnia magna were determined as > 100 mg/L (95% confidence limits: not available).